FastQCFastQC Report
Wed 23 Aug 2023
SRR8668648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51751141
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT44255148.551529327633569No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC27680105.348693664551281No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG16583643.20449746219122No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG11592232.2399950563408835No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT9915771.9160485756246417No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA8388811.6209903468601783No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG5558821.0741444328734704No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG5258181.0160510277444899No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA4605680.8899668511656583No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATA4271600.8254117527572966No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA4216210.8147086071010492No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG4058600.7842532399430575No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG3546660.6853298171725334No Hit
CATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG3426240.6620607649983988No Hit
TGGTGGGGCTGCATGGGACCTTCGCTGTAGGCACCATCAATAGATCGGAA3414270.6597477725177113No Hit
TAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC3354810.6482581707715391No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT3044850.5883638391663674No Hit
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC2980540.5759370600157396No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT2974790.5748259734022096No Hit
TGGTGGGGCTGCATGGGACCTTCGTCTGTAGGCACCATCAATAGATCGGA2867000.5539974471287502No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2663520.5146785072816076No Hit
TTCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA2506080.4842559896408854No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA2469600.4772068696997425No Hit
TCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGATC2317080.4477350557352928No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAA2100950.4059717253383843No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATAG2017450.38983681538538445No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATC1909310.3689406577528407No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAGA1778650.34369290524435003No Hit
TTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGGA1754220.3389722363802568No Hit
TTATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1718420.3320545145081922No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA1442620.2787610035496608No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1441390.27852332763059273No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1383120.26726367250530764No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG1308710.2528852455639577No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1293590.2499635708515103Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGGGACCCACATGGCGTGTAAGCCCTGTAGGCACCATCAATAGATCGG1283150.247946224026249No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCTCTGTAGGCACCATCAATAGAT1234050.238458510508976No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG1222600.23624599890464248No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT1217910.23533973869291114No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG1211480.2340972540103029No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1189270.22980556119525944No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG1139080.22010722430255208No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGA1109070.21430831834219846No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA1108560.21420976979038975No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1075740.20786788063281542No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT975550.18850792101376082No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAAT968980.1872383837875188No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT943470.18230902387253645No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA930730.17984724240186317No Hit
TGGTGGGGCTGCATGGGACCTTCGTTCTGTAGGCACCATCAATAGATCGG902000.17429567398330406No Hit
GCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGG894280.17280391943435605No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAA865500.16724268939307058No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCCTGTAGGCACCATCAATAGATC856160.1654378982677889No Hit
TAACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAG847840.16383020424612474No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATA841140.1625355468007942No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC794000.15342656889439404No Hit
TTTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGA784990.15168554447910626No Hit
CTCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA782280.15116188452733825No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC778400.1504121426037737No Hit
AAGTCCCCCCTCGGACACCACTGTAGGCACCATCAATAGATCGGAAGAGC772880.14934549945478495No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC762530.14734554355043109No Hit
AACCCTGACGGCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC753250.14555234637242107No Hit
AACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAGA726570.14039690448564215No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA723070.13972059089479785No Hit
TGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC720100.13914669050485284No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT690110.13335164919358977No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG686090.13257485472639144No Hit
GGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGG680110.13141932464832032No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT676530.13072755246111384No Hit
CTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG675510.13053045535749636No Hit
AGGTGCGGCTGGATCACCTCCTGTAGGCACCATCAATAGATCGGAAGAGC641410.12394122865812755No Hit
TACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCAT632390.12219827191829451No Hit
TCAGCGGTACTAACCATCCAAAACCACCTGTAGGCACCATCAATAGATCG631660.12205721222648985No Hit
ACTCCCCCAGGGCCGGAAGGCAGCAAGGGCTGTAGGCACCATCAATAGAT620990.11999542193668734No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA612820.11841671278320222No Hit
AAGGGGGACCCACATGGCGTGTAAGCCCTGTAGGCACCATCAATAGATCG608770.11763412134236809No Hit
ATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT606490.11719355134604664No Hit
ATAACCATCCTAAGGTAGCGAAATTCCTCTGTAGGCACCATCAATAGATC593860.11475302544537135No Hit
GATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG572270.1105811367521346No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAATA556560.1075454548915163No Hit
TAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCA552880.10683435945885715No Hit
TATCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGA541240.10458513368816352No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC535570.10348950567099574No Hit
CAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA533940.10317453677011683No Hit
TTGGGGTATGGTGTAATTGGCAACACAGCTGACTGTAGGCACCATCAATA521230.10071855227307937No Hit
GCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC519430.10037073385493085No Hit
TTGGTGGGGCTGCATGGGACCTTCGCTGTAGGCACCATCAATAGATCGGA518400.1001717044267681No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)