FastQCFastQC Report
Fri 14 Jul 2023
SRR8668649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50602266
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT31171166.16003243807303No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC29729005.8750333433684565No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG14574152.8801378183340645No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT12107292.3926379107212314No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA8327971.645770171636187No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG8160511.6126767919839795No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATA6495181.2835749292334062No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA6309461.246873015528593No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA5870091.1600448881083705No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG5716241.129641111328888No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG5156241.0189741305260913No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG4683810.9256126988463323No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT3463370.6844298237553236No Hit
TGGTGGGGCTGCATGGGACCTTCGCTGTAGGCACCATCAATAGATCGGAA3454830.6827421522980809No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATAG3194480.6312918872052093No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG2839650.5611705214940375No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAGA2837200.5606863534530252No Hit
TGGTGGGGCTGCATGGGACCTTCGTCTGTAGGCACCATCAATAGATCGGA2720870.53769726438733No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT2653320.5243480598279926No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2386220.4715638623772303No Hit
TCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGATC2287370.452029164069451No Hit
CATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG2236060.44188930195339476No Hit
TAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC2154840.4258386373448177No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA2118030.4185642595531196No Hit
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC2030550.40127649619485417No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAA2009640.3971442701795212No Hit
ATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1758150.34744491481863676No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA1752210.3462710543436928No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA1703400.33662524124907767No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG1598460.3158870395250679No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1588300.3138792243019315No Hit
TTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGGA1489490.2943524307784952No Hit
TTCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA1456370.2878072693424441No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATC1397680.27620897451509385No Hit
AACCCTGACGGCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1354440.26766390264024936No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA1337790.26437353615745196No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1329120.2626601741510943No Hit
TGGTGGGGCTGCATGGGACCTTCGTTCTGTAGGCACCATCAATAGATCGG1310710.2590219971572024No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT1203300.2377956750000089No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG1197630.23667517181938058No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT1195990.23635107566131525No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCTCTGTAGGCACCATCAATAGAT1194510.23605859864062215No Hit
TATCCTGAGAGGTGATGCATAGCCGCTGTAGGCACCATCAATAGATCGGA1137230.2247389474613647No Hit
AGGGGGACCCACATGGCGTGTAAGCCCTGTAGGCACCATCAATAGATCGG1043190.20615479947083795No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1031990.20394145985478201No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1020090.20158978651272258No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG1015380.20065899815632765No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC980460.19375812142483895No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAAT970080.19170682988781568No Hit
TTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA955330.18879194066131347No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA927070.18320721052294378Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT903460.17854141156445444No Hit
GCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC871220.17217015538395058No Hit
TTATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA867900.17151405828347688No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC851560.16828495387933814No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCCTGTAGGCACCATCAATAGATC838450.16569416081090121No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC834920.1649965635926265No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATA834610.1649353015139678No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT818590.16176943538457347No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAA813780.1608188850673209No Hit
TTTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGA785120.15515510708552063No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG779250.15399507998317705No Hit
AAGTCCCCCCTCGGACACCACTGTAGGCACCATCAATAGATCGGAAGAGC774980.1531512442545557No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA711150.14053718463912268No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA702270.13878232251496406No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGA695690.1374819854905312No Hit
TTATCCTGAGAGGTGATGCATAGCCGCTGTAGGCACCATCAATAGATCGG680120.1344050481849963No Hit
TGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC678630.13411059496821742No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG658540.1301404170319171No Hit
AACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAGA657440.12992303546248304No Hit
TAACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAG619330.12239175217963558No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT604650.11949069632573371No Hit
GGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGG604390.11943931522750385No Hit
GCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGG597880.11815281157567133No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG589930.11658173568748877No Hit
AGGTGCGGCTGGATCACCTCCTGTAGGCACCATCAATAGATCGGAAGAGC588040.11620823462727935No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAATA588030.11620625843119359No Hit
ATCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGAT582100.11503437415233539No Hit
CTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG557070.11008795534966755No Hit
AGGTGCGGCTGGATCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA546220.10794378259661336No Hit
TCAGCGGTACTAACCATCCAAAACCACCTGTAGGCACCATCAATAGATCG545040.10771059145849317No Hit
TATCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGA544710.10764537698766297No Hit
TACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCAT528200.10438267725006622No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTCTGTAGGCACCATCAATAGATCGG512350.10125040645412994No Hit
ACTCCCCCAGGGCCGGAAGGCAGCAAGGGCTGTAGGCACCATCAATAGAT511290.10104092966903894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)