FastQCFastQC Report
Wed 23 Aug 2023
SRR8668650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37096836
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT23500456.334893358560283No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC20428525.506809259959529No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG12108783.2641004747682523No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG8121702.189324178482499No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA6867861.851333089431131No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT5708411.5387862188570476No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATA5623161.5158058223617776No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG3389480.9136843907658324No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG3381540.9115440465057452No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA3209690.8652193410780369No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG2581750.6959488404887144No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAGA2578410.6950484941626828No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA2564190.6912152831578412No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATAG2540050.6847079896517321No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA2393240.6451331860215788No Hit
CATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG2127030.5733723490596341No Hit
TCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGATC1994570.5376657998541978No Hit
TGGTGGGGCTGCATGGGACCTTCGTCTGTAGGCACCATCAATAGATCGGA1940740.5231551283780644No Hit
TGGTGGGGCTGCATGGGACCTTCGCTGTAGGCACCATCAATAGATCGGAA1836170.494966740559761No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT1782670.4805450254571576No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG1602000.4318427587732819No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT1444910.3894968293252826No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC1288190.34725063884154433No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1278290.34458194763564204Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC1262070.3402096070942546No Hit
TTATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1256980.3388375224237452No Hit
TTCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA1168680.31503495338524284No Hit
TAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC1164890.31401330291348833No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1155140.311385046422827No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC1133870.3056514038016611No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1127440.30391810234166605No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA1126880.3037671460714332No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATC1110530.29935976211017024No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT1079140.29089812403408205No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT1075210.28983873449476927No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1060630.2859084801733496No Hit
AACCCTGACGGCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1059550.2856173502236148No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA1059080.28549065478252644No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAA1047560.28238526865202196No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1039240.28014248977999096No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG976430.2632111266847663No Hit
AGGGGGACCCACATGGCGTGTAAGCCCTGTAGGCACCATCAATAGATCGG902510.24328489901402914No Hit
ATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT888180.2394220358846776No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT885810.2387831673838707No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCTCTGTAGGCACCATCAATAGAT800330.2157407709918981No Hit
TGGTGGGGCTGCATGGGACCTTCGTTCTGTAGGCACCATCAATAGATCGG777980.20971599842099742No Hit
TTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGGA742620.20018418821486555No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG737620.19883636437350075No Hit
AACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAGA726040.1957148043568999No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGA717520.19341811253121424No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG701610.18912933706799145No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG693620.1869755145694905No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA677730.18269213040163318No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAA601710.16219981671752276No Hit
ACTCCCCCAGGGCCGGAAGGCAGCAAGGGCTGTAGGCACCATCAATAGAT584680.15760912871383426No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG577590.15569791450677897No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAATA567230.1529052235074711No Hit
TAACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAG555830.14983218514915936No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCCTGTAGGCACCATCAATAGATC551350.1486245349872965No Hit
AAGTCCCCCCTCGGACACCACTGTAGGCACCATCAATAGATCGGAAGAGC550600.14842236141109177No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA550030.14826870949317617No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA548830.14794523177124863No Hit
TATCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGA525020.14152689463866946No Hit
AAGGGGGACCCACATGGCGTGTAAGCCCTGTAGGCACCATCAATAGATCG518870.13986907131379073No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG508030.13694698922571186No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT505050.13614368621625844No Hit
TACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCAT497670.13415429822640398No Hit
CAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA490310.132170301531915No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC474150.127814134876624No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATA473330.12759309176664016No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT468440.12627492004978538No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAAT465740.12554709517544838No Hit
TAGACTGTGAAGCTCACACGCCAGTGTGGGTGGAGTCGCTGTAGGCACCA462670.1247195313368504No Hit
TTTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGA455360.12274901288077507No Hit
GCGCCGTTAGCTCAGTTGGTAGAGCAGCTGACCTGTAGGCACCATCAATA450080.12132571090429382No Hit
ATCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGAT449980.12129875442746653No Hit
GGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGG442240.11921232312103382No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG438850.1182984985565885No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC435630.11743050000274956No Hit
TTGGGGTATGGTGTAATTGGCAACACAGCTGACTGTAGGCACCATCAATA422180.11380485386947826No Hit
GCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGG408350.11007677312426321No Hit
CTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG407990.10997972980768494No Hit
TCCTCTCGCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA389590.10501973807146249No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACCTGTAGGCACCATCAATA380170.10248043795433119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)