FastQCFastQC Report
Fri 14 Jul 2023
SRR8668653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38544039
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG9058952.3502856044744043No Hit
AGTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAA6736361.7477047488458592No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5374111.3942778544822456Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA4485191.1636533472789399No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT2857580.741380528387282No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA2575570.6682148697493794No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC2519440.6536523066511011No Hit
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT1600200.41516147282852217No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT1549560.4020232544907917No Hit
TGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCAAT1545420.400949158441854No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG1355030.3515537123652246No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1350250.3503135724826347No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA1312200.3404417476850311No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGTCTGTAGGCACCATCAATA1218920.31624085893022263No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG1153300.2992161771110703No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG1149420.2982095363695538No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1145910.2972988897193675No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC1127560.29253810167637073No Hit
TTGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATC1025260.26599703264102653No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC1000610.25960175061051594No Hit
ATGTGCGTCCATGATGAATCAGCGGTACTAACCACTGTAGGCACCATCAA997790.2588701199684859No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT990820.25706179884261743No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA943420.24476417741275117No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGATCG892670.2315974202911117No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTACTGTAGGCACCATCAATAGA824340.21386964661383825No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC821630.21316655475571725No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG770970.2000231475481851No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGCTGTAGGCACCATCAATAGATCG763290.19803062154435863No Hit
TGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCA754260.19568784682892212No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG711940.18470819832867022No Hit
TATTCCCGTACCCGTGTATGTGCGTCCCTGTAGGCACCATCAATAGATCG690130.17904973580999126No Hit
TGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAAG661260.17155960225133646No Hit
TGTGGCCTGTGTGCAGGTGGTGCATGGCTGTCGTCCTGTAGGCACCATCA658880.17094212674494233No Hit
AACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAAT637190.16531479744507316No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG634580.16463764993596028No Hit
TCGTCAGCTCGTGTCGTGAGATGTTGGGCTGTAGGCACCATCAATAGATC594880.1543377433797221No Hit
AGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGATC575120.14921114001571034No Hit
AGCAACGCTGCGGTGAATACGTTCCCGGGCCTTGTCTGTAGGCACCATCA574880.14914887357809076No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT560530.14542585949542028No Hit
TGTGGCCTGTGTGCAGGTGGTGCATGGCTGTCTGTAGGCACCATCAATAG558400.14487324486154657No Hit
TTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAAG553850.14369277698167543No Hit
ATGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA515440.13372755252764248No Hit
ATTCCCGTACCCGTGTATGTGCGTCCCTGTAGGCACCATCAATAGATCGG513940.13333838729252012No Hit
ACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTGTAGGCACCATCAATA513570.13324239320118994No Hit
GTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAA511420.13268458969751457No Hit
AGGAGGGATAAGGCCCCCCGCAGACCACGGGACTGTAGGCACCATCAATA504450.13087626857164605No Hit
AGAAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGA497690.12912243057869466No Hit
TGTGTGGTGTAGTGGTGTGTTCTGGACCCGCTGTAGGCACCATCAATAGA485600.12598575878360854No Hit
TTGAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAG482970.12530342240469403No Hit
AACTCCGTGATAGCTGGTTCTCCCCGAAATGCCTGTAGGCACCATCAATA474260.12304366960608358No Hit
AGGGAGTCAGATAGGTAAATCCGTCTGGCTGTAGGCACCATCAATAGATC467070.1211782709123971No Hit
AGTGTGTGGTGTAGTGGTGTGTTCTGGACCCGCTGTAGGCACCATCAATA463420.12023130217359941No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA456730.11849562522495373No Hit
AAGCCTCTAGCGAGGACATACACGGCCCGTACCCCCTGTAGGCACCATCA456010.11830882591209499No Hit
ACGTAGGGTCCGAGCGTTGTCCGGAATTCTGTAGGCACCATCAATAGATC454290.11786258310915469No Hit
AGGAGGGATAAGGCCCCCCGCAGACCACGGGATCTGTAGGCACCATCAAT438560.11378153701017166No Hit
AAAAGCCGGTCTCAGTTCGGATCGGGGTCTGCCTGTAGGCACCATCAATA433870.11256474704168912No Hit
TGTGGCCTGTGTGCAGGTGGTGCATGGCTGTCCTGTAGGCACCATCAATA431920.11205883223603007No Hit
AAGAGTGCGGCAGTGAGACGGCGGGGGATCTGTAGGCACCATCAATAGAT427110.11081090904873772No Hit
AACACCGGTGGCGAAGGCGGGTCTCTGGGCAGTCTGTAGGCACCATCAAT422890.10971605752059352No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAGAT419270.10877687208649825No Hit
AGTGCGGCAGTGAGACGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG417290.10826317397613676No Hit
AACGCTGCGGTGAATACGTTCCCGGGCCTTGTCTGTAGGCACCATCAATA415050.10768202055835405No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTAGTCTGTAGGCACCATCAATAGA413970.10740182158906594No Hit
AACTCGGAGGAAGGTGGGGATGACGTCCTGTAGGCACCATCAATAGATCG412220.10694779548142322No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTCTGTAGGCACCATCAATAGATCGG410910.10660792450941636No Hit
ACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGAT405260.10514206879045551No Hit
TAGTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGA399000.10351795254254491No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG397630.10316251496113316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)