FastQCFastQC Report
Fri 14 Jul 2023
SRR8668654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36950576
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA21128095.717932516126406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA12396573.354905753025338No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG8599332.3272519486570387No Hit
AGTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAA6430531.740305753285145No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT2461220.6660843392536019No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT2323670.628858938491243No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC1941740.525496544356981No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1606200.4346887583024416No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA1397730.3782701519997956No Hit
ATGTGCGTCCATGATGAATCAGCGGTACTAACCACTGTAGGCACCATCAA1274630.344955380397859No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1244820.3368878471610294No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1181640.3197893315654944Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG1177330.3186229086117629No Hit
TGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAAG1151840.3117245046464228No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1117970.3025582063998136No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC1044680.2827236035508621No Hit
TGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCAAT1029980.27874531644648787No Hit
AAGAGTGCGGCAGTGAGACGGCGGGGGATCTGTAGGCACCATCAATAGAT1025950.27765467038998254No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG966310.2615141912808071No Hit
ATTCCCGTACCCGTGTATGTGCGTCCCTGTAGGCACCATCAATAGATCGG958790.2594790403267327No Hit
GTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAA909610.24616936959250652No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGTCTGTAGGCACCATCAATA851600.2304700202778977No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT797330.21578283380480995No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC785650.21262185466337521No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG768330.20793451230638463No Hit
AACTCGGAGGAAGGTGGGGATGACGTCCTGTAGGCACCATCAATAGATCG760620.2058479413149067No Hit
TATTCCCGTACCCGTGTATGTGCGTCCCTGTAGGCACCATCAATAGATCG755420.2044406560807063No Hit
AGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGATC742460.20093326826623759No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTACTGTAGGCACCATCAATAGA739640.20017008665845964No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC668530.1809254610807691No Hit
TCGTCAGCTCGTGTCGTGAGATGTTGGGCTGTAGGCACCATCAATAGATC644060.17430310152675293No Hit
TGTGGCCTGTGTGCAGGTGGTGCATGGCTGTCGTCCTGTAGGCACCATCA643910.17426250676038174No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGATCG638420.1727767383111971No Hit
TGTGTGGTGTAGTGGTGTGTTCTGGACCCGCTGTAGGCACCATCAATAGA631290.17084713374968769No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG616780.16692026668271692No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA610120.16511785905583717No Hit
AGGGAGTCAGATAGGTAAATCCGTCTGGCTGTAGGCACCATCAATAGATC591470.16007057643702224No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT575360.15571069852875907No Hit
ATTCCCGTACCCGTGTATGTGCGTCCTGTAGGCACCATCAATAGATCGGA529960.14342401590708626No Hit
AAGTGCACAAGGGAGCTTGACTGCGAGACGGACCTGTAGGCACCATCAAT519770.1406662781116051No Hit
AGAAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGA512110.13859323870891754No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG504370.1364985487641654No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG495680.1341467586323959No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTCTGTAGGCACCATCAATAGATCGG485970.13151892408930244No Hit
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT483750.1309181215470092No Hit
TGTGTGCGGGGTTGTGGGACCTATCTGTAGGCACCATCAATAGATCGGAA478220.1294215278267922No Hit
AGGTCCCAAGGGTTGGGCTGTTCGCCCATTCTGTAGGCACCATCAATAGA476600.1289831043499836No Hit
AGTGCGGCAGTGAGACGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG470350.12729165575118506No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA460840.12471794756325313No Hit
TATTCCCGTACCCGTGTATGTGCGTCCTGTAGGCACCATCAATAGATCGG453630.12276669245967911No Hit
AGTGTGTGGTGTAGTGGTGTGTTCTGGACCCGCTGTAGGCACCATCAATA451050.12206846247809505No Hit
GGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA440950.11933508154243658No Hit
GTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC440570.11923224146762963No Hit
TGTGGCCTGTGTGCAGGTGGTGCATGGCTGTCTGTAGGCACCATCAATAG439910.11905362449559649No Hit
TTGAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAG437940.11852047989725517No Hit
TGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGATCGG436680.1181794838597374No Hit
ATGATGAATCAGCGGTACTAACCACTGTAGGCACCATCAATAGATCGGAA436540.1181415954111243No Hit
ATGTGCGTCCATGATGAATCAGCGGTACTAACCCTGTAGGCACCATCAAT433810.11740277066316909No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC418330.1132133907736648Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGGAGCTTGACTGCGAGACGGACCTGTAGGCACCATCAATAGATCGGA416570.11273707884824312No Hit
AGGCGTCTGGGGGGAACGCGGGGAAGTGACTGTAGGCACCATCAATAGAT414790.11225535428730528No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGCTGTAGGCACCATCAATAGATCG412700.11168973387586706No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT412260.11157065589451162No Hit
TGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATAG401620.10869113379991695No Hit
ACCGCGTGGAGGCCCGAACCCACTTAGGCTGTAGGCACCATCAATAGATC388550.10515397649010938No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA378430.10241518291893474No Hit
AAGAGTGCGGCAGTGAGACGGCGGGGGACTGTAGGCACCATCAATAGATC376300.1018387372364642No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)