FastQCFastQC Report
Fri 14 Jul 2023
SRR8668655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27279234
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC17988066.594048791839242No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA17857246.546092899822627No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA8441343.0944197333400196No Hit
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT5519522.0233412712395076No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA4543111.6654096665617517No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG3129511.1472132978513987No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT3022531.1079966541582509No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG2546590.9335269458079357No Hit
AACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAAT2197300.8054844941760462No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG1920930.7041729984060403No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT1895460.6948362259732073No Hit
ACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGAT1706630.6256150740889572No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1580220.5792757963804995No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1252110.4589974923782684No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1151100.4219693265580698No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA1059170.38826969994831967No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1022120.3746879402845402Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG1006510.36896563884455114No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT1002460.36748099305134446No Hit
TCCGGTTCAGGGACAGTGCCTGGTGGGTAGCTGTAGGCACCATCAATAGA957680.35106557610818545No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG949090.3479166607097546No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT935820.3430521546169515No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTACTGTAGGCACCATCAATAGA935500.34293484927032775No Hit
AGTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAA922920.3383232828311821No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG913490.3348664408978639No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA872960.3200089855895514No Hit
ACCACCGTGACCGCACCTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG858960.31487687667476294No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC842550.30886131186821447No Hit
TGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATAG828760.30380618458714787No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC798790.2928198057174186No Hit
TGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCA786270.2882302340307649No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG777410.28498234224612023No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG759880.2785562087263887No Hit
ATCTACCCATGGCCAGGGTGAAGCGCGGGCTGTAGGCACCATCAATAGAT710120.2603152273263978No Hit
GTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGATCGG691160.25336488553894143No Hit
GTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC675450.2476059261781324No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATA658490.24138874280707442No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA649600.23812985364618378No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG626800.22977184769924258No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT623970.22873442854003892No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC612620.22457375452697828No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG610500.22379660660559603No Hit
AGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGATC592880.2173374809571266No Hit
TGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCAAT582880.21367168887513485No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG577130.21156385842798958No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA559470.20509006961119217No Hit
AGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGATC549300.20136195906380658No Hit
GGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA528330.1936747930678699No Hit
GCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC502680.18427203637756104No Hit
AGAAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGA487940.1788686588487052No Hit
AGTGTGTGGTGTAGTGGTGTGTTCTGGACCCGCTGTAGGCACCATCAATA482090.17672417048074002No Hit
GTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAA481640.1765592098370504No Hit
TGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAAG467640.17142710092226196No Hit
TGTGGAGGGAGCCGTCGAAGGTGGGATCGGCGACTGTAGGCACCATCAAT465140.17051065290176404No Hit
TTGAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAG464010.17009641839649897No Hit
TCGTCAGCTCGTGTCGTGAGATGTTGGGCTGTAGGCACCATCAATAGATC458900.16822319864260118No Hit
ATGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA450430.16511827274915417No Hit
TGTGTGGTGTAGTGGTGTGTTCTGGACCCGCTGTAGGCACCATCAATAGA443920.16273184210377753No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCCTGTAGGCACCATCAATAG437050.16021344294344922No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC435010.1594656213587229No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG402740.14763611031013554No Hit
TCGGCTCGTCGCATCCTGGGGCTGGCTGTAGGCACCATCAATAGATCGGA400330.14675265441837554No Hit
AGCTCGTAGGTGGTTTGTCGCGTTGTTCGTGCTGTAGGCACCATCAATAG369170.13533004629088927No Hit
CCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG360440.13212980980331046No Hit
TGGAGGGAGCCGTCGAAGGTGGGATCGGCGACTGTAGGCACCATCAATAG360260.13206382554583462No Hit
AACCCTGACGGCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC357570.13107772747577884No Hit
AGGCGTCTGGGGGGAACGCGGGGAAGTGACTGTAGGCACCATCAATAGAT353340.12952709742509633No Hit
TCTACCCATGGCCAGGGTGAAGCGCGGGCTGTAGGCACCATCAATAGATC353050.12942078945471855No Hit
TGTGGAGGGAGCCGTCGAAGGTGGGATCGGCGCTGTAGGCACCATCAATA351680.1289185759394857No Hit
TCCGGTTCAGGGACAGTGCCTGGTGGGCTGTAGGCACCATCAATAGATCG348200.12764288029495258No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGCTGTAGGCACCATCAATAGATCG345560.12667511118530675No Hit
TGAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGA340000.12463693078771934No Hit
TGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGAT336380.12330991405403832No Hit
TAGCCTAGTAATAGGTGCAGGGAACTGGCACTGTAGGCACCATCAATAGA335390.12294700063792115No Hit
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC315750.11574738498888934No Hit
ATCCGGTTCAGGGACAGTGCCTGGTGGGTAGCTGTAGGCACCATCAATAG299120.10965117275653707No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC298480.1094165620632896No Hit
TAGCCTAGTAATAGGTGCAGGGAACTGGCACCTGTAGGCACCATCAATAG285070.10450073488133868No Hit
TATCCGGTTCAGGGACAGTGCCTGGTGGGTAGCTGTAGGCACCATCAATA282790.10366493428664457No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGATCG279110.10231592280047161No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGTCTGTAGGCACCATCAATA277160.1016010933444832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)