FastQCFastQC Report
Fri 14 Jul 2023
SRR8668656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19708813
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA13165506.680006553413439No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC12164766.172243858623044No Hit
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT10197475.174066038375827No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG5800522.943109765159373No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA4797102.4339872725972893No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA2471031.2537690626015885No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG2076721.0537012046336833No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1932870.9807135518511441No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1712780.8690426967874728No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT1692600.8588036225215593No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG1324270.6719176847433683No Hit
AACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAAT1299440.6593192598661319No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1293760.6564373004097203No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG1116390.5664420277365259No Hit
ACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGAT1063280.5394946920446199No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT891180.452173350064258No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT865790.43929078833920643No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG790030.4008511319276305No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC785970.39879113978097No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG748680.3798706700398446No Hit
TGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCAAT741790.3763747720372607No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA732850.3718387302167817No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG729370.37007302266250125No Hit
ACCACCGTGACCGCACCTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG669460.33967545381855313No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG644850.32718865413152987No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC605870.3074106999746763No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA582930.29577123695881635No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG527020.2674032170278342No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTACTGTAGGCACCATCAATAGA519940.2638109154518844No Hit
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC478210.2426376464173667No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA472300.23963898789845944No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG461910.23436723459703024No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC450230.22844095177116958No Hit
AGTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAA447750.22718263144513068No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT436280.22136289993720065No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA426430.21636513573902194No Hit
TTGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATC395490.20066657489723No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCCTGTAGGCACCATCAATAG376900.19123424632422054No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG376530.1910465130497712No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG373460.1894888342590698No Hit
CATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG362160.18375535857994085No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT354350.17979266432737476No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGA353240.1792294645040267No Hit
TTTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGA352240.1787220772757852No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA345710.17540883867536822Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGATCGG328440.16664626124363754No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGATCG325310.16505813921924167No Hit
AACCCTGACGGCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC305530.15502201984462483No Hit
CAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA291030.1476649050351231No Hit
CCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG280590.14236778237228187No Hit
TTCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA277680.14089128553809913No Hit
GTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAA276670.1403788244375752No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT274700.13937927159793945No Hit
AGCTCGTAGGTGGTTTGTCGCGTTGTTCGTGCTGTAGGCACCATCAATAG271570.13779114957354358No Hit
GCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC265240.1345793884187749No Hit
TTGAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAG262460.13316885192426353No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT261540.13270205567428134No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC254290.12902349826953047No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC249980.12683665931580965No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATA249830.1267605512315734No Hit
TGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCA237730.12062116576985128No Hit
TGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCG233280.11836329260417663No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC230130.11676502283521589No Hit
ATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT224530.11392365435706352No Hit
TAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC217060.11013347176209952No Hit
TCGTCAGCTCGTGTCGTGAGATGTTGGGCTGTAGGCACCATCAATAGATC214150.10865697492791677No Hit
TACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGA212820.10798214991435558No Hit
TCAGCGGTACTAACCATCCAAAACCACCGTCTGTAGGCACCATCAATAGA211420.10727180779481747No Hit
ATCTACCCATGGCCAGGGTGAAGCGCGGGCTGTAGGCACCATCAATAGAT210290.10669846022690459No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAGAT207420.10524225888185149No Hit
TGGTGGGGCTGCATGGGACCTTCGTTCTGTAGGCACCATCAATAGATCGG205720.10437970059384094No Hit
AGAAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGA204610.10381650077049287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)