FastQCFastQC Report
Fri 14 Jul 2023
SRR8668657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26799766
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC14729095.495977091740278No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA13743925.12837313579529No Hit
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT8011512.989395504423434No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG7292622.721150624971875No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6074382.2665794917761595Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA3574831.3339034378135988No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA3209351.1975291127541934No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT2538650.9472657335888679No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG2301460.8587612294823768No Hit
AGTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAA2238310.8351975909043385No Hit
ACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGAT1812690.67638277140181No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG1556320.580721488389115No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG1462520.5457211827894318No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1432060.5343554119091936No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1426100.5321315118945441No Hit
AACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAAT1362950.5085678733165058No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1238110.461985377036501No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT1188100.4433247663431091No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT1182130.4410971349525962No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1146030.42762686808534073No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT1143400.42664551623323876No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG1089270.40644757868408254No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC1042290.38891757487733286No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG973840.36337630709163654No Hit
ACCACCGTGACCGCACCTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG873210.3258274717771789No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA806230.30083471624341795No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG735290.2743643358677087No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTACTGTAGGCACCATCAATAGA731570.27297626404648456No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG724830.27046131671448176No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT700830.26150601464206813No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG684040.2552410345672421No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA668480.24943501372362728No Hit
TTGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATC654130.24408048935949667No Hit
ATCTACCCATGGCCAGGGTGAAGCGCGGGCTGTAGGCACCATCAATAGAT649340.24229316032087744No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG608320.22698705652877715No Hit
TGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCAAT601160.2243153914105071No Hit
TCCGGTTCAGGGACAGTGCCTGGTGGGTAGCTGTAGGCACCATCAATAGA554770.20700553877970426No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG539530.20131892196372161No Hit
TGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCA537360.2005092134013409No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC532190.19858009207990848No Hit
TTGAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAG515950.19252033767757523No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA507280.18928523480391582No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG493220.18403892033982686No Hit
TCTACCCATGGCCAGGGTGAAGCGCGGGCTGTAGGCACCATCAATAGATC487180.18178516931826944No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC477440.17815080922721488No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA463140.17281494174240178No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGCTGTAGGCACCATCAATAGATCG450840.1682253494302898No Hit
TCGTCAGCTCGTGTCGTGAGATGTTGGGCTGTAGGCACCATCAATAGATC448740.16744176049895362No Hit
GTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGATCGG422540.15766555573656874No Hit
TCGGCTCGTCGCATCCTGGGGCTGGCTGTAGGCACCATCAATAGATCGGA420940.15706853559840783No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGATCG411800.15365805805916366No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA410720.15325506946590506No Hit
GTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAA404500.1509341536788045No Hit
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC404160.1508072868994453No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA394910.14735576422570257Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCACCGTGACCGCACCTTTCGGGGTGTGGCCTGTAGGCACCATCAATAG386970.14439305179007905No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGA378470.14122138230609924No Hit
TACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGA370570.13827359537392975No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC367120.13698627070102032No Hit
TTTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGA345350.12886306544616846No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC326560.12185181019864129No Hit
CCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG326080.121672704157193No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGACTGTAGGCACCATCAATAGA315830.11784804389709969No Hit
TGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCG289060.10785915071049501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)