FastQCFastQC Report
Fri 14 Jul 2023
SRR8668658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25490944
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT15572286.108945984895656No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC15513586.085918199027859No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA11959424.691634801755478No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA7773893.049667364221584No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG6880262.699099727338462No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA4246191.6657641239178904No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG3010631.1810586536143972No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2965291.1632719447345694Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG2487990.9760289771928415No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA2370670.9300047891517865No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT2040320.8004097455158976No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG1978220.7760481526302047No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1737450.6815950009540643No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG1447620.5678957986020446No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1332300.5226562029244582No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT1155160.4531648572920642No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT1106450.4340561102797919No Hit
ACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGAT1084530.42545697797617854No Hit
AACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAAT1084430.42541774835800505No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC887090.3480020198545805No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC856530.33601344854078374No Hit
TGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCAAT843830.3310312870327596No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA814060.3193526297025328No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG812140.3185994210336031No Hit
TTGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATC792720.31098102918432524No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG770320.3021935947134794No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG749010.2938337630807239No Hit
TGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCCTGTAGGCACCATCA728370.28573676988973024No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA719860.28239832938317233No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTACTGTAGGCACCATCAATAGA717040.2812920541506819No Hit
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC695750.27294006844156105No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG653280.25627924960331006No Hit
ACCACCGTGACCGCACCTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG630010.24715051745435557No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG622840.24433775383132145No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT616960.2420310522827244No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACCTGTAGGCACCATCAATAGATCG611780.2399989580613413No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC598510.23479318772972865No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC574590.2254094630626469No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA512420.20102040944423244No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATA509430.1998474438608472No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG473350.1856933976238777No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGATCG413960.1623949273906843No Hit
AGTGCGGCAGTGAGACGGCGGGGGCTGTAGGCACCATCAATAGATCGGAA407710.15994307625484563No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG400870.1572597703717838No Hit
TTTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGA382660.15011605690240423No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG363190.1424780502440396No Hit
GTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAA360080.14125800911884628No Hit
AACCCTGACGGCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC357580.14027726866451082No Hit
ACCACCGTGACCGCACCTTTCGGGGTGTGGCCTGTAGGCACCATCAATAG355650.13952013703376384No Hit
CATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG354690.13914353269929902No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT354260.1389748453411533No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT342780.13447128517484483No Hit
TTCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA319980.12552693223130537No Hit
TTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAGAT316700.12424020075521723No Hit
CAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA309060.12124305792676802No Hit
AGCTCGTAGGTGGTTTGTCGCGTTGTTCGTGCTGTAGGCACCATCAATAG306900.1203956981742222No Hit
AAGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATC304020.11926588517082773No Hit
ATGTGCGTCCATGATGAATCAGCGGTACTAACCACTGTAGGCACCATCAA303270.1189716630345271No Hit
TCCTGCACCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCACA302980.11885789714182417No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGTGCTGTAGGCACCATCAATA301950.11845383207463794No Hit
GTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG297940.11688072438588387No Hit
GTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAGATCGG291370.11430333847189025No Hit
GCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC290650.11402088522104163No Hit
TCCGGTTCAGGGACAGTGCCTGGTGGGTAGCTGTAGGCACCATCAATAGA289220.11345990168116174No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGA283390.11117281494165143No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC279410.10961147613834936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAGTATCTCGTATGC279040.10946632655110772TruSeq Adapter, Index 1 (97% over 37bp)
ATCTACCCATGGCCAGGGTGAAGCGCGGGCTGTAGGCACCATCAATAGAT276730.10856012237130173No Hit
TGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGAAG275910.10823843950227971No Hit
CCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATAG267690.10501376488842469No Hit
TTGAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAG267400.10489999899572176No Hit
TGGTGGGGCTGCATGGGACCTTCGTCTGTAGGCACCATCAATAGATCGGA255470.10021990554763291No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)