FastQCFastQC Report
Tue 27 Jun 2023
SRR870723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR870723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768017
Total Bases286.3 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG198900.5278638604868291TruSeq Adapter, Index 11 (100% over 49bp)
CACGACCTCAGCTCAGACGAGAAGAGATCGGAAGAGCACACGTCTGAACT64240.17048755353279987Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACGACCTCAGCTCAGACGAGAAGAAGATCGGAAGAGCACACGTCTGAAC37970.10076918442777727Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)