FastQCFastQC Report
Tue 27 Jun 2023
SRR870743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR870743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases304 Mbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTC1296723.2418000000000005No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTG781961.9549Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACG758481.8961999999999999No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCTG612941.53235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG580761.4519TruSeq Adapter, Index 7 (100% over 49bp)
ATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTG547361.3684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGT510141.27535No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCT461761.1544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACG407411.018525No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTC401641.0041No Hit
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGT397440.9936No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTC397380.99345No Hit
CGCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGT395530.988825No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCAGATCGGAAGAGCACACGT334770.836925No Hit
CCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGTC328080.8201999999999999No Hit
CGGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGT310660.77665No Hit
CCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCT283260.7081500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTG272790.681975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCAGATCGGAAGAGCACACG258870.6471750000000001No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGT247200.618No Hit
CTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAA241660.60415Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCT236760.5919Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTC228180.57045No Hit
CCCCTGTCCGCGGCTGGAGCGTCGCAGGCCAGATCGGAAGAGCACACGTC225170.562925No Hit
GGCCCCCGGCGCGGAGAGCCATTCGTGAGATCGGAAGAGCACACGTCTGA216580.54145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC211680.5292No Hit
AGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGA205740.51435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTAGATCGGAAGAGCACACGT201200.503No Hit
ACCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCT200870.502175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTC197110.49277499999999996No Hit
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTCT184010.460025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGAGATCGGAAGAGCACACGTC181960.4549No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTC167230.418075No Hit
AAGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTC159760.39940000000000003No Hit
GGATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTC159620.39905No Hit
GGCCCCCGGCGCGGAGAGCCATTCGTGACAGATCGGAAGAGCACACGTCT147760.3694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTGA146930.367325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCT142350.355875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTTCGAACGCACATTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTG136170.34042500000000003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTC135780.33945No Hit
GATCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCT132050.330125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACG128550.321375No Hit
CTCCGGGGCCGCGGGTGAGTGTCCTCCGCGCCCAGATCGGAAGAGCACAC125750.31437499999999996No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCTG119830.29957500000000004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACAC118590.296475No Hit
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAGATCGGAAGAGCACACGT118550.296375No Hit
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTC118350.295875No Hit
CGCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACG116520.2913No Hit
TAAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC115010.28752500000000003No Hit
ACCGCCTGGGAATACCAGGTGCTGTAAAGATCGGAAGAGCACACGTCTGA104240.2606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGCCTGGGAATACCAGGTGCTGTAAGAGATCGGAAGAGCACACGTCT103220.25805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGT100650.251625No Hit
CCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTCT100640.2516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTC96550.241375No Hit
CTTCGCCGCTTGCTCTCCGGGGCCGCGGGTGAAGATCGGAAGAGCACACG96120.2403No Hit
GGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACGT89660.22415No Hit
TCTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCT86830.217075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCTG84510.211275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGT81030.20257500000000003No Hit
CCATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGT80830.202075No Hit
GGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAAC80600.20149999999999998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGAGATCGGAAGAGCACACGTCT72490.181225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA72010.180025Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCCAGATCGGAAGAGCACACG71100.17775No Hit
AATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAAC70080.17520000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGT69770.174425No Hit
ACGATGCCGACCCGCGATCCGGCGGCGTTAGATCGGAAGAGCACACGTCT69700.17425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCCGCGATCCGGCGGCGTTTAGATCGGAAGAGCACACGT69340.17335No Hit
CCCCCTGTCCGCGGCTGGAGCGTCGCAGGCCAGATCGGAAGAGCACACGT69310.17327499999999998No Hit
GAGCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACG69310.17327499999999998No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAAGATCGGAAGAGCACACGTC68840.1721No Hit
CGAGGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCT66880.16720000000000002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCCGCGGGTGAGTGTCCTCCGAGATCGGAAGAGCACACGTCTG66080.1652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTGA65990.16497499999999998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCTG64010.160025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCATTGAATACCCCAGCTAGGAATAATGGAGATCGGAAGAGCACACGTCT63020.15755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGTC60780.15195No Hit
GCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA59650.149125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGA58680.1467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTGACGCAAACGAACCCGGAGAGATCGGAAGAGCACACGTCTGA57860.14465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC57490.143725No Hit
GCGCCGCGACCGGCTCCGGTTCGGCTTGGAGATCGGAAGAGCACACGTCT57340.14335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGAGATCGGAAGAGCACACGTC57020.14254999999999998No Hit
TCTCCGATAGGCCCCGGGTAACCGGGAAGATCGGAAGAGCACACGTCTGA56560.1414Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACG54440.1361No Hit
AGGGTCAGGGGGCGAAGGTGGCCCGTCGGAGATCGGAAGAGCACACGTCT53420.13355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGACGATCGAACTCGGTCGTTTAAGATCGGAAGAGCACACGTCTGAA53250.133125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGAGTCGGGTCCAGATCCCCGAGCCCGGAGAGATCGGAAGAGCACACG53170.132925No Hit
GCCGCTTGCTCTCCGGGGCCGCGGGTGAGTGAGATCGGAAGAGCACACGT52730.131825No Hit
AGCCCGCGGACGGTGTGAGGCCGGTGTCGGCAGATCGGAAGAGCACACGT52600.1315No Hit
AACTCTTAGCGGTGGATCACTCGGCTCGAGATCGGAAGAGCACACGTCTG52030.130075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAACTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG51830.129575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCGAGATCGGAAGAGCACAC51470.12867499999999998No Hit
AATACCCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAA50410.126025Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGACGATCGAACTCGGTCGTTTAGATCGGAAGAGCACACGTCTGAAC50240.1256Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAGAGATCGGAAGAGCACACGTC49680.1242No Hit
GGGAGTCGGGTCCAGATCCCCGAGCCCGGAGCAGATCGGAAGAGCACACG49670.12417500000000001No Hit
AACCCCGCCCGTGATGGGGACCGGGGATTAGATCGGAAGAGCACACGTCT49130.12282499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGCGGACGGTGTGAGGCCGGTGTCGAGATCGGAAGAGCACACGTCT48710.12177500000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGTTCGGCTTGGAAGGAGATCGGAAGAGCACACG48610.121525No Hit
AGTGCGGTGACGCAAACGAACCCGGAGAAGATCGGAAGAGCACACGTCTG48120.1203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCTG47540.11885000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT46740.11685000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACG46490.11622500000000001No Hit
CCCCCGGCGCGGAGAGCCATTCGTGACAGAGATCGGAAGAGCACACGTCT45290.113225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGAATACCCCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCT44710.111775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTTTCGAACGCACATTGCGGCCCCGGAGATCGGAAGAGCACACGTCTG43790.109475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCCGCGATCCGGCGGCGTAGATCGGAAGAGCACACGTC43340.10835No Hit
CCTTTCGAACGCACATTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCT43130.107825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCCCCGGCGCGGAGAGCCATTCGTGACAGAGATCGGAAGAGCACACGT42960.10740000000000001No Hit
GGGACCTGTGGCGTACGGAGGGCCGCAGATCGGAAGAGCACACGTCTGAA42900.10725000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGGCGCGGAGAGCCATTCGTGACAGGAGATCGGAAGAGCACACGTCT42340.10585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACTCTAGATAACCTCGGGCCGATCGCGCAGATCGGAAGAGCACACGTC41470.10367499999999999No Hit
GCCGAGAAGACGATCGAACTCGGTCGTTAGATCGGAAGAGCACACGTCTG41150.102875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGAAGACGATCGAACTCGGTCGTTTAGAGATCGGAAGAGCACACGT40600.1015No Hit
TGCGGCGCAATGAAGGTGAGAACGGGGGAGATCGGAAGAGCACACGTCTG40450.10112499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCGCGACCGGCTCCGGTTCGGCTTGAGATCGGAAGAGCACACGTCT40270.100675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCAGATCGGAAGAGCACACGTCTG40140.10035000000000001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCGACCGGCTCCGGTTCGGCTTGGAAAGATCGGAAGAGCACACGTCT40060.10015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTACGGAGGGCCGCCTCTCTCGGCGAGATCGGAAGAGCACACGTCTGAA40050.10012499999999999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCCCTGCTTCTGGCGTCAAGCGCCCCGGAGATCGGAAGAGCACACGTC40020.10005000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)