FastQCFastQC Report
Fri 14 Jul 2023
SRR8711056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8711056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62921178
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA29838374.742182353928593No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG13135882.0876722937386836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA10412951.6549197473702735Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACAC7242741.1510814371593614No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTC7131071.1333338355489784No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6697701.0644587741189462No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC6004540.9542955473592691No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC5345050.8494834600839799No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGAGATCGGAAGAGCACACGT4648950.7388529820595539No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG4012930.6377709584521765TruSeq Adapter, Index 8 (100% over 49bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3814340.6062092480213895No Hit
GCGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACAC3719240.5910950999677724No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACACG2995150.4760161991881335No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA2927050.46519313417813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2907380.4620670007163566No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2787380.4429955205225179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2705940.430052342630966No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAAGATCGGAAGAGCACAC2521070.4006711381023413No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA2379640.37819380940388625No Hit
GCGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAAGATCGGAAGAGCACA2279530.3622834270521763No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1878650.2985719688846258No Hit
GTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACG1850070.29402977801845986No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC1802220.2864250252911667No Hit
AGCGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACA1788050.28417300133827755No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGT1647140.2617783157206624No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC1632300.25941980933669107No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG1585700.252013717861417No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCGAGATCGGAAGAGCACACG1559800.24789745671958016No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1440600.22895311972703372No Hit
TGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAAC1379010.21916468251754598Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1364860.2169158371446892No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1265470.20111988367414227No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1052470.16726800633007854No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1007110.16005898681680752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAA1003130.15942644939037853Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCGAGTGTTACAGCCCCCCGGCAGCAGCAGATCGGAAGAGCACACGTC956850.15207121519562142No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCAGATCGGAAGAGCACAC927810.14745591698871244No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAGATCGGAAGAGCACAC913660.14520707161585564No Hit
GCGTTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACAC908980.14446328388829593No Hit
CGTTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACACG902290.14340004886748942No Hit
GCGCTCGCCGAATCCCGGGGCCGAGGGAGCCGAGATCGGAAGAGCACACG889750.14140707918723328No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA832680.1323370010650468No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG811060.12890095605012353No Hit
GCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAAGATCGGAAGAGCACACG804270.1278218281291555No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCGAGATCGGAAGAGCACACGTC801630.12740225556489104No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACG792120.12589084075952933No Hit
CGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGA760210.12081941631798439Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGGAGATCGGAAGAGCACACGTC746310.1186103031955314No Hit
GTTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACACGT728420.11576706335663328No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGAGATCGGAAGAGCACACGT712960.1133100209916604No Hit
TCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACACGTCT711630.11309864541951202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT700850.11138539078209883No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCAGATCGGAAGAGCACACG690810.109789743605881No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC688460.109416260452085No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC684840.10884093746623752No Hit
AGCGCTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC679280.10795729221725633No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG654780.10406353167768093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACA648820.1031163148280536No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT629890.10010778882747554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)