FastQCFastQC Report
Mon 17 Jul 2023
SRR8711058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8711058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46470194
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG531938211.446868502421143TruSeq Adapter, Index 4 (100% over 49bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA8454211.8192758136538014No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC7600231.6355064065366287No Hit
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCA3703920.7970528377824289Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC3433500.7388606985372171No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG3187630.6859515155026036No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA3011010.6479443576241579No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG2822940.6074732547920932No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCAC2358100.5074435454261285No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2250990.48439436254559215No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC2151910.4630731690080743No Hit
CGCCGTCGGGCCCGGGGGGCCGTCGTCACGCGAGATCGGAAGAGCACACG2146120.46182720907082936No Hit
GCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACAGATCGGAAGAG2030520.43695104866573187No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAGATCGGAAGAGCAC1978260.42570513047567654No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCT1937760.41698986666593213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAG1895610.40791953655282787No Hit
GTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGATCGGAAGAGCACACGT1888010.40628407964038193No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1825290.3927872562787235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG1761320.37902144329330756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCAC1699270.3656687983699831No Hit
CGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACAGATCGGAAGAGCA1586110.34131770571046033No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG1487980.32020094428699825TruSeq Adapter, Index 4 (100% over 50bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTC1381330.29725074958800474No Hit
CGCGGGCGGGCGCGACCCGCTCCGGGGACAGATCGGAAGAGCACACGTCT1234500.26565415242294876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1229780.2646384476036403TruSeq Adapter, Index 4 (100% over 50bp)
GAGACGGGCCGGTGGTGCGCCCTCGGCGGACAGATCGGAAGAGCACACGT1199390.25809877187084695No Hit
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCAGATCGGAAGA1196020.2573735758451966No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1192290.25657091080790406No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1181270.2541994982848576No Hit
GGACGGCGCCGGACGCGCACGCCCCGCCGGGCCAGATCGGAAGAGCACAC1144110.24620297474979339No Hit
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCAGATCGGAAGAGC1142500.24585651611439366No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG1138670.24503233190720058No Hit
CTCGCTTCTGGCGCCAAGCGCCCGTCCGCGAGATCGGAAGAGCACACGTC1113920.23970633735680122No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1094400.23550579539220345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTC1078120.2320024745323852No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA1049090.22575545951024006Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1048380.22560267340394577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCAGATCGGAAGAGCA1011160.2175932383669412No Hit
AGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCAGATCGGAAGAGCACACG979370.21075229425553937No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT947110.2038102100456047Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCAGATCGGAAGAGCAC946590.20369831036212158No Hit
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG898480.1933454377229413No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC898030.19324860145838857No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC871050.18744272941920578TruSeq Adapter, Index 4 (100% over 50bp)
GAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACAGATCGGAAGAGC856500.184311690198668No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT812000.17473565959289947Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA781540.1681809204411757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGAAGCGGGGCGCGCCGACCGGGGTCGCGCAGATCGGAAGAGCACACG744130.16013059898135998No Hit
CGAAGAAGCGGGGCGCGCCGACCGGGGTCGCGCAGATCGGAAGAGCACAC724960.1560053741114143No Hit
CGCGGCCCCGGGGGCGGACCCGGCGGGGGGGACAGATCGGAAGAGCACAC723030.15559005413233265No Hit
GGCGAGTGCTGCTGCCGGGGGGGCTGTAACAGATCGGAAGAGCACACGTC709620.15270433344866172No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGAGATCGGAAGAGCACAC668100.14376957410593122No Hit
ACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGCACACGTCTGAACT653230.1405696735417115Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC635880.1368360975639568No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAAGATCGGAAGAGCACACG591850.12736120705672113No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA590700.1271137366028642No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT555360.11950886195999096No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGT555300.11949595045805059No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAG553940.1192032897474024No Hit
CCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCAC552200.1188288561911319No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACACG539680.11613465611957634No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG539660.11613035228559623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT524920.1129584266422473Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGCGCCGCGCGGAACCGCGGCCCCGGGGGCGGACCAGATCGGAAGAGC521090.11213424243505417No Hit
CGCGGGCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCAC519070.11169955520306199No Hit
CGCGCAGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTC518990.1116823398671415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAAGAAGCGGGGCGCGCCGACCGGGGTCGCAGATCGGAAGAGCACACGT518260.1115252499268671No Hit
GCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAAGATCGGAAGAGCACACG492120.10590013891484938No Hit
CGGGGGCGGGGAGCGGGGCGTGGGCAGATCGGAAGAGCACACGTCTGAAC491350.10573444130661472Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTCAGATCGGAAGAGCACAC490110.10546760359984725No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC487730.10495544735621289Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCGGTTGGCCCCGGATAGCCGGCTCCCCGAGATCGGAAGAGCACACGT483980.10414847848494026No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC477400.10273251710548056No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG473340.10185883880751605No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT469100.10094642600373047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)