FastQCFastQC Report
Tue 25 Jul 2023
SRR8711059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8711059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229763132
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA68176042.9672314877741135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG48293612.101886824906269Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA30852491.3427955012382056No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTC23462971.021180804586177No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG17158240.7467795137820458TruSeq Adapter, Index 8 (100% over 49bp)
TGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAAC14031360.6106880541652784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACAC13205040.5747240597329601No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12660490.5510235645638745No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA12519340.5448802813151067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA9983500.43451270502353706Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCC8382080.3648139685003946Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC7835590.34102903854914374No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT7193390.3130785142674674Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC7165270.3118546451569088No Hit
GGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAA6709280.2920085542705781Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG6350090.27637549787578625No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGAGATCGGAAGAGCACACGT5635810.24528782972892274No Hit
GAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAAC5133620.22343097238072118Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGT5125720.223087140020358No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5036100.21918660126899733No Hit
GTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACG4994170.217361678374057No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC4954000.21561335610623553No Hit
AGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACT4541000.19763832258345085Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG4192690.18247879733812125No Hit
TCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCC4129100.17971116445261548Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3810330.16583731109654268Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCAGATCGGAAGAGCACACGTCTGAACT3507890.15267418969549912Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACT3500240.15234123810603348Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGA3437770.1496223510741488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3197410.1391611427023897No Hit
ATGTACACGGAGTCGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTC3108150.13527627226112152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG3005050.1307890423429639Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2993300.1302776461107781No Hit
GTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACTC2909110.12661343770331265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG2823530.12288873220965668No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACACG2799340.12183590881760786No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2622960.11415930733395468No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2543890.11071793711447143Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGAA2493650.10853133739489589Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGAACTC2489060.10833156644121651Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACAC2372360.1032524225862311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)