FastQCFastQC Report
Tue 25 Jul 2023
SRR8711060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8711060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227706616
Total Bases11.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA66557552.922951962010625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG47307992.077585220448755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA30349811.3328470877631418No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTC23192951.018545284604291No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG16988130.7460534216537652TruSeq Adapter, Index 8 (100% over 49bp)
TGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAAC13859160.6086410769900511Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACAC13072760.5741054093922331No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA12271710.5389263700620803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT11940430.5243778248410665No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA9853770.4327397320769986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCC8262450.36285506961290925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC7768210.34114994708805474No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT7127060.3129931016145793Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC7079720.3109141106378745No Hit
GGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAA6540560.2872362742415881Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG6315920.27737094823806085No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGAGATCGGAAGAGCACACGT5578060.24496697100799214No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGT5056970.22208269960851731No Hit
GAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAAC5030250.22090925983459347Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACG4970020.21826418956575247No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4945250.2171763863022759No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC4883480.2144636851482611No Hit
AGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACT4467130.1961791922637856Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG4156420.18253400243759277No Hit
TCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCC4037550.17731368859304467Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3797820.166785667747133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCAGATCGGAAGAGCACACGTCTGAACT3476980.15269560722820633Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACT3471650.1524615340996504Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGA3344970.14689823505172112Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3131160.1375085210523703No Hit
ATGTACACGGAGTCGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTC3060530.1344067227278104Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2952260.12965192017082192No Hit
ACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG2942080.12920485367012788Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACTC2859930.12559714119154097Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG2804270.123152767770261No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACACG2772920.12177599617922388No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2526660.11096120281371183No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2517200.1105457559476445Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGAA2460950.10807547199243434Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGAACTC2448040.1075085143771141Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAAAGCACACGTCTGAA2369810.10407295324260583Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GCGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACAC2353770.10336853804897789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)