FastQCFastQC Report
Tue 25 Jul 2023
SRR8711061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8711061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225739923
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA65506972.9018779279020133Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG46251762.0488958880348336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACA30068061.3319779505727924No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTC22784681.0093332050972659No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG16846780.7462915631454344TruSeq Adapter, Index 8 (100% over 49bp)
TGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAAC13588140.6019378326801326Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACAC12892320.5711138654016462No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGA11996190.5314164123286247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT11277370.49957357343477077No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA9799210.4340929096533802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCC8072750.3576128623026065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC7528480.33350237299407604No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT7133570.31600834735821187Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC6913290.306250215208942No Hit
GGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAA6360660.2817693882176083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG6245410.27666395544929817No Hit
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGAGATCGGAAGAGCACACGT5505240.24387533790378763No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGT4987970.22096091527416709No Hit
GTACACGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACG4936310.21867244102851935No Hit
GAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAAC4925790.2182064180114033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACAGATCGGAAGAGCACACGTC4822990.21365250487836834No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4786380.2120307270592982No Hit
AGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACT4350960.1927421584174103Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCGCGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG4038920.178919171510482No Hit
TCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCC3988120.17666879420349585Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3761160.16661474629811052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGAGCTCAACCAGATCGGAAGAGCACACGTCTGAACT3436370.1522269501261414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACT3429090.15190445511049458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGAGTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGA3225930.1429047178331854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3080790.13647519495255608No Hit
ATGTACACGGAGTCGAGCTCAACAGATCGGAAGAGCACACGTCTGAACTC3029600.13420754112687458Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAACTCCAG2871250.1271928315488971Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2866860.1269983599666595No Hit
GTCGAGCTCAACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAACTC2820780.12495707283465317Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAAAGCACACGTCTGAA2788290.12351780593103154Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACACG2756000.12208739878058698No Hit
GGTCGCGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG2713300.12019584147727383No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2493080.1104403672539571Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2448010.1084438218754952No Hit
ACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGAACTC2399800.10630817837215263Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTACACGGAGTCGAGCTCAACCCGCAGATCGGAAGAGCACACGTCTGAA2378620.1053699305106966Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCTCGCCGAATCCCGGGGCCGAGGGAGCCGGAGATCGGAAGAGCACAC2333780.10338357384838835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)