FastQCFastQC Report
Fri 14 Jul 2023
SRR8728404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8728404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31634587
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG558586117.657448791729127No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA21136256.6813737761141No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT21034826.649310768621699No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA18271245.775716306964905No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG14939444.722501988092969No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA14273574.512014018074584No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12681854.008855876639073No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA10991663.474570412441294No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT8480412.6807399129313745No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3504221.1077179544022497No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCCTGTAGGCACCAT3447191.089690217861861No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2197890.694774361998151No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1866650.5900661829408426No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAAT1553830.4911807446703825No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1244180.39329737416834304No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA1215080.38409858172006484No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1126240.35601539542779553No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGA1120800.35429575862646795No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1118600.35360031727298985No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1098300.34718329023862393No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1081290.3418062641374139No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAG1004080.31739943372739465No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC997940.31545852013177855No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC962620.30429352531139414No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG933250.2950093832424618No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC912720.2885196509756868No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA878120.27758225514371343No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT833330.26342370140631205No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA832710.26322771338851364No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT796820.2518825360356372No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT786150.2485096454712685No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA779910.24653712090503976No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT774030.24467839583301657No Hit
ATGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG737170.2330265920651975No Hit
TTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA735090.23236908387645458No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG686110.21688603047038357No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC670750.2120305853842821No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCA670340.2119009804047703No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA657870.20795909236937404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCCTGTAGGCACCATCAA639970.20230072862971152No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA629410.19896261013301672No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA615410.19453707424724717No Hit
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT606870.1918374973569277No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA594630.18796831455394059No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT554900.17540927592953878No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT550790.17411006503735926No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT529730.1674527946263373No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA523680.1655403309042726No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAGAT519940.16435808060335985No Hit
AGATAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCG516600.16330227418489768No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA510280.16130446084217887No Hit
TTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCCTGTAGGCACCATCA485010.15331636856836472No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA482710.15258931624427402No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA481180.15210566839390063No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG475290.15024378222481613No Hit
CCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCCTGTAGGCACCAT465840.1472565455019217No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC461110.1457613465919438No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA458570.14495842793838276No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCTGTAGGCACCATC457820.14472134565878797No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA456590.14433253072025248No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC439490.13892705474549105No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA429890.13589240156667765No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACCTGTAGGCACC429760.1358513073048812No Hit
GCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCCTGTAGGCACCAT416200.13156485968980725No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA393260.12431330303126764No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC380870.12039670377236157No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG375890.1188224774358521No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCA374740.11845895127380673No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG369990.11695743016970635No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT349500.11048034229117643No Hit
TGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGAT346630.10957310743459367No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAGAT343180.10848252894845758No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT339250.10724021780338085No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT335790.1061464782201835No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG335270.10598210117299778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)