FastQCFastQC Report
Mon 24 Jul 2023
SRR8728405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8728405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28953611
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG23712658.189876558056955No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT22135047.645001516391169No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA15880575.484832271871029No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG15317825.2904696412478565No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11395593.9358095955630543No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA6210172.144868907715863No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA4353271.5035326681704744No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4299511.4849650359673618No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG4250581.4680655894699972No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC3872561.3375050179405945No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3846181.3283938918706892No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3471761.1990766885691737No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA2728490.9423660489187342No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2668460.9216328837187182No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA2568120.8869774481670006No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1876730.6481851262006664No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGAT1874880.6475461730835577No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC1783600.6160198809053558No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG1736350.5997006729143387No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1619670.5594017271282673No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1451250.5012328168669531No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA1442160.49809331209153845No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1320720.4561503572041498Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1275280.4404562871277092No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAGAT1268470.4381042488966229No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1149900.3971525347909109No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCA1144900.39542563447440116No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGA1113430.38455652388228884No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCCTGTAGGCACCAT1099730.3798248170150521No Hit
AGATAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCG895640.3093361998957574No Hit
TCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAG891620.30794777204128354No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC848330.2929962691009422No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAGAT832290.2874563728855789No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA807230.2788011484992321No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA789640.2727259131857508No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT779520.2692306669451351No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT776380.268146173546367No Hit
ACCCCCCTCCGTCGCCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCA767860.2652035354070344No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA762870.26348008889115765No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA724250.25014151084643643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG719710.24857348535904555No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG708640.24475012805829297No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCCTGTAGGCACCATCAATAGATCGGA708170.24458779942854106No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC703640.24302322774178323No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC676510.23365306662440133No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACCTGTAGGCACC669160.23111452315913206No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA643910.22239367656075784No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC634740.21922654138027894No Hit
TTAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCGGA633210.218698109883427No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA619060.21381098198770437No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG561030.1937685769142923No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA553740.1912507562528211No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA547270.1890161472432575No Hit
TTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA547210.18899542443945938No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA526000.18166991329682505No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT525630.18154212267340333No Hit
CCCCCCGGGCCGCGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC523240.18071666432211167No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAAT505890.17472432022382287No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA503180.17378834025227458No Hit
TACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGA497560.17184730429651762No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT467230.16137192697656952No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA466730.16119923694491856No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATCG460560.15906824195434552No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT447500.15455757832762207No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCTGTAGGCACCATCAATAGATC434620.15010908311229296No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCCTGTAGGCACCATCAATAGATCGGA408450.14107048685568097No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA406560.1404177185360403No Hit
ATAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCGGA395130.136470024412499No Hit
TTAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG393030.13574472627956494No Hit
TCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAA389180.13441501303585243No Hit
TTGGGGAAGGTGGCTCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG380850.1315379971085472No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA377900.13051912592180642No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT364310.12582541086153295No Hit
TGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCG362800.125303886965947No Hit
TGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG350670.12111442679809438No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA349890.12084503034871885No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG348130.12023716143730742No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG340430.11757773494988243No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG338630.11695605083593891No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT328620.1134987964022864No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT318090.10986194433571687No Hit
TTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA312250.1078449247660335No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG312150.10781038675970331No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT311850.10770677274071273No Hit
TCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC309360.10684677638309087No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC308670.10660846413941252No Hit
TCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA299010.10327209272791571No Hit
TCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGA294400.10167989063609373No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA292260.10094077730062755No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)