FastQCFastQC Report
Mon 17 Jul 2023
SRR8728406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8728406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48127980
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG23453524.873156945294609No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT21640654.496480010172876No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG17653123.667953651908931No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12306662.557069712878039No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11387022.365987519110505No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA10527702.187438575232121No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC6703831.3929173840248439No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6526361.3560427842598006No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5836911.212789317149816No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGAT5759571.1967196628655514No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA5748661.1944527902480013No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5601631.1639029936431988No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA4839601.0055689019152685No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA4531220.9414939085330405No Hit
ATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGGAAGA4468470.9284557548436481No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3444590.7157146425010981No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3238280.6728476865224762Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC3095950.6432744528234927No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA2650160.5506485001032664No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGA2576820.5354099631856563No Hit
AGATAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCG2468530.5129095382769024No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC2184250.4538420270287679No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACCTGTAGGCACC2062780.4286030703968876No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1938690.4028197318898487No Hit
TTAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCGGA1884580.3915767917124301No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA1682510.3495908201424618No Hit
ATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACA1675990.348236098834815No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1674780.347984685831402No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1632030.33910211897528214No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA1625060.3376538969638867No Hit
AGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1607990.33410710360168866No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG1592100.3308054898626537No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA1577290.32772827781261543No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA1532310.31838236302458567No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCTGTAGGCACCATCAATAGATC1524550.316769995333276No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1470680.30557692219785665No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA1441720.2995596324632781No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCCTGTAGGCACCATCAATAGATCGG1422560.2955785802770031No Hit
TTAACCTCGGGCCGATCGCACGCCCCCTGTAGGCACCATCAATAGATCGG1376040.2859126853028114No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1344810.27942373646265645No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1289140.26785666051224255No Hit
ATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGGAAGAG1274500.26481477095028716No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA1255550.2608773524257615No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATCG1254130.26058230576059915No Hit
TATGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGA1252080.2601563581101887No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1240980.2578500074177225No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCCTGTAGGCACCAT1192870.24785374329028562No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA1191630.2475960969066227No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1178860.24494275471357826No Hit
TACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGG1178060.24477653124024734No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA1160960.2412235044977994No Hit
AGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAG1156700.24033836450231238No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1086440.22573978795702626No Hit
TCTAGACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1083810.2251933282884509No Hit
ACCGCCCGTCCCCGCCCCTTGCCTGTAGGCACCATCAATAGATCGGAAGA1077210.22382198463347103No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGAT1040000.2160905153301676No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA1031710.2143680245877762No Hit
TCGCGGCGCCTCGCCTCGGCCGGCGCCTGTAGGCACCATCAATAGATCGG1030670.214151934072446No Hit
TGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCG1012230.2103204830121688No Hit
CTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATC1003530.2085128027396953No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1000980.20798296541845304No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCCTGTAGGCACCATCAATAGATCGGA989720.20564337003132066No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG930220.19328049920233512No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAGAT928220.19286494051900788No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT895290.18602276679802476No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG872230.18123137517926163No Hit
TATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGGAAGAGCA868170.1803877910521073No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA849390.17648569501566447No Hit
TCCCCCGCCTCCCCTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA844820.17553614342426174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG818860.17014219171467407No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA815710.16948768678843368No Hit
TTCCCCCGCCTCCCCTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC808160.1679189527588733No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC798790.16597206032748515No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC794990.16518249882916342No Hit
ATGCGTGCATTTATCAGATCAAAACTGTAGGCACCATCAATAGATCGGAA794240.16502666432291568No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA788200.16377167709926743No Hit
ACCCCCCTCCGTCGCCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCA781220.16232137729445534No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA724920.15062340035879337No Hit
TGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGG716140.14879909773898675No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG709540.14742775408400685No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG705420.1465717031963527No Hit
TCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA700130.14547255047895216No Hit
TATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGGAAGAGCAC699000.14523775982287226No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC684370.14219794805433345No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCTGTAGGCACCATCAATAGATCGGA667240.1386386879316356No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC661410.1374273343697367No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA645850.13419428781345072No Hit
CCCCCCGGGCCGCGGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA645580.13413818739120154No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT642090.13341303748879552No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT619900.1288024138972797No Hit
TTGGGGAAGGTGGCTCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG616580.12811258648295648No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT598920.12444320330917691No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG598760.12440995861451074No Hit
TCGCGGCGCCTCGCCTCGGCCGGCCTGTAGGCACCATCAATAGATCGGAA595520.12373675354752059No Hit
AAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAG591550.122911869561116No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT588390.12225528684145896No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGA586980.12196231796971325No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA580610.12063876356331597No Hit
TATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAG578410.12018164901165601No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATAGATCGG568350.11809138883451997No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG558910.11612995184921537No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA546050.11345790951542117No Hit
TCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAG542280.11267458139734932No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG538100.11180606374919537No Hit
TAGATAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATC534150.11098533534962406No Hit
CCCCCCGGGCCGCGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC533490.11084820098412607No Hit
TAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCGGAA530390.11020408502496884No Hit
ATGACTCTCTTAAGGTAGCCAAATGCCTCGTCTGTAGGCACCATCAATAG528530.1098176154494745No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG523150.10869976259132422No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA491930.1022128915445859No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC486270.10103686047076982No Hit
TCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC485380.1008519368566892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)