FastQCFastQC Report
Fri 14 Jul 2023
SRR8728407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8728407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26114013
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA17150736.567634779074362No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG10265023.9308473959938675No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA9257903.5451847251512048No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8975513.437047381419317No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT8220633.1479765289233788No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA6667142.5530890254209493No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA6654862.548386569310508No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5367622.055455819831291No Hit
CCCCCCGGGCCGCGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3867471.4809941313883852No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3173141.2151100637041117Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCCGGGCCGCGGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2314830.886432123626499No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA1943800.7443513181983941No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1939910.7428616965152005No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1819880.6968978685887918No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1776630.6803358794376031No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA1773150.6790032615822011No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA1695000.6490768002604579No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGAT1526000.5843605883170848No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCA1499880.5743582956782628No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1499380.5741668275955901No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1451210.5557207925109021No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA1407460.5389673352770407No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAGA1321460.5060348250573361No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1227870.4701958293426598No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGG1175920.45030229555296614No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1121170.42933654050030534No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1010100.3868038206153915No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATCG981120.3757063305436817No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC943370.3612504903018927No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGA921160.3527454780695713No Hit
ACCCCCCTCCGTCGCCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCA905710.34682911431498487No Hit
GGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCG832960.31897050828610674No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC807090.30906394968862116No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA754770.2890287295177497No Hit
AAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC732060.2803322492027556No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC731960.28029395558622106No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA723110.2769049705229143No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG703000.2692041242378182No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC697970.26727795532613086No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT664620.25450703421186166No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG664390.2544189588938322No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC651990.24967055044354922No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA642530.2460479743193817No Hit
TGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCG604670.23155001109940476No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA593560.22729559030241733No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG592100.22673650350101304No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA577420.22111500059374253No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT574410.21996236273605285No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT573890.21976323593007324No Hit
TTGGGGAAGGTGGCTCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG573030.21943391082787622No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA562430.21537478747521493No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG529860.20290255656991515No Hit
AGATAACCTCGGGCCGATCGCACGCCCCTGTAGGCACCATCAATAGATCG517470.1981579774812856No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC516460.19777121195428676No Hit
CTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGAA508310.19465028220672173No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT499100.19112344012389057No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAAT474480.18169555173308674No Hit
TTCCCGTGGATCGCCTCAGCTCTGTAGGCACCATCAATAGATCGGAAGAG471290.18047398536563491No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA468160.1792753951681038No Hit
CGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGG466650.17869716155843224No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG454030.17386450715177326No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT450680.17258167099786617No Hit
GCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC414770.15883043330031274No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC409730.15690043502697193No Hit
AGCCGGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT409220.15670513758264576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG405680.15534954355732303No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATC402700.15420839378459372No Hit
CCCCCGGGCCGCGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA399710.15306341465021098No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA397570.1522439312563718No Hit
TGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG396900.1519873640255904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGAAGGTGGCTCGGGGGGGGCCTGTAGGCACCATCAATAGATCGGA393950.15085770233782145No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA386810.14812353811725526No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGGAA384500.1472389555753074No Hit
CGCGGCGCCTCGCCTCGGCCGGCCTGTAGGCACCATCAATAGATCGGAAG372400.14260542797462802No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA363730.13928537142108338No Hit
TCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGAAGA350720.1343033719099397No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG347720.1331545634139035No Hit
TGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGG343340.13147730300969063No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG335690.1285478413447983No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATCG333390.12766708816450387No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCCTGTAGGCACCATCAATAGATCGGA327810.1255303043618765No Hit
TAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT323720.12396409544561382No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA322100.12334373885775428No Hit
TGGACGAGGCGCCGCCGCCCCTGTAGGCACCATCAATAGATCGGAAGAGC318110.1218158235580261No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCCTGTAGGCACCATCAATAGAT314560.12045640017104993No Hit
AAGTGGGAGGCCCCCGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGC311720.119368861461469No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC310060.11873318742699561No Hit
TTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG305690.1170597563844362No Hit
CCGGGTTTCGCCCCCGCGGCGTCGGGCCTGTAGGCACCATCAATAGATCG300500.11507231768629356No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCATCAATAGATC293350.11233432410407394No Hit
ATGCGTGCATTTATCAGATCAAAACTGTAGGCACCATCAATAGATCGGAA292470.11199734027856997No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATAGAT285630.10937805690760742No Hit
TCTTCTGGCGCCAAGCGTCCGTCCTGTAGGCACCATCAATAGATCGGAAG284560.10896831521068784No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATCG284070.10878067648966859No Hit
TTCCCGTGGATCGCCTCAGCCTGTAGGCACCATCAATAGATCGGAAGAGC280290.10733317778466296No Hit
CGCGGCGCCTCGCCTCGGCCGGCGCCTGTAGGCACCATCAATAGATCGGA278080.10648688885924963No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGAT276400.10584355610146937No Hit
TCCCCCGCCTCCCCTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA274720.10520022334368907No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAAT271450.10394802208300961No Hit
ACCCCCCTCCGTCGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC267930.10260008678099379No Hit
TGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGG266580.1020831229577775No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT263880.10104919531134492No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAG261880.10028332298065411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)