FastQCFastQC Report
Fri 14 Jul 2023
SRR8730291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8730291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123466744
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length10-290
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1536189512.442131785705794No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT63037555.105629901441314No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT31493022.5507289639062645No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC30145172.441561915652364No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC18883551.529444236417217No Hit
CGACTGCATAATTTGTGGTAGTGGGG17249991.397136543910156No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA15292311.2385772479753738No Hit
CCCGGATAGCTCAGTCGGTAGAGCATCAGA14523851.1763370061820049No Hit
CGACTGCATAATTTGTGGTAGTGGGGG14457521.1709647093309596No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC14267001.1555338334669294No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC10399450.8422875393879342No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT10394980.8419254985779815No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA10274450.8321633556644208No Hit
GCAGAGTGGCGCAGCGGAAGCGTGCTGGGC10229260.8285032607646964No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC8302780.672470961087303No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG6849300.5547485726196846No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG5996770.48569920982122927No Hit
CCGGATAGCTCAGTCGGTAGAGCATCAGA5053930.40933532676621No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC4649660.3765920967349718No Hit
GCAGAGTTGCGCAGCGGAAGCGTGCTGGGC4506070.36496224440809744No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA4346590.35204540584629007No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGAGA4073920.3299609164391668No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG3972100.3217141613453417No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA3817680.30920714974066216No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT3674560.29761536434458824No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC3649690.295601056750958No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG3493770.2829725549415963No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG3435710.2782700740856987No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3434410.2781647825749742No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG3270850.26491749065643133No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3178870.2574677113053212No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGG3138010.2541583181297791No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC3054840.24742209124750222No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG2742280.2221067723305314No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG2741020.22200472055859838No Hit
AGAGTGGCGCAGCGGAAGCGTGCTGGGC2514150.20362973206777044No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2374400.1923108946648824No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC2349450.1902901075936691No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2316890.18765296021736833No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC2254850.18262812535171413No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG2245550.18187488608268473No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT2165720.17540917738950013No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG2089930.16927068231425946No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT2081260.16856846893119656No Hit
TCCGGGGCTACGCCTGTCTGAGCGTCGCT2072770.16788083437269552No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCA2010480.1628357511395943No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA1961280.15885087242601945No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGG1858440.15052150399301045No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGGGA1839960.14902474467132623No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG1828450.1480925098340651No Hit
CTTCCGGGGCTACGCCTGTCTGAGCGTCGCT1819790.1473911063857001No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1778450.14404283634465972No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA1750560.14178392847226942No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1746040.14141783798882718No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1714660.13887626290687635No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1706440.13821049658521814No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG1676220.1357628739282215No Hit
GCATAATTTGTGGTAGTGGGG1669560.13522345741943273No Hit
ACTGCATAATTTGTGGTAGTGGGGG1661160.1345431122732126No Hit
CCCGGATAGCTCAGTCGGTAGAGCATCA1656530.13416811250809368No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA1629020.13193998215422284No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGAGA1594330.1291303186872734No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGT1581800.12811547051082842No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT1531360.12403015989471626No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATG1520300.1231343721188598No Hit
GCAGAGTCGCGCAGCGGAAGCGTGCTGGGC1483680.12016839125521929No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGAG1471350.11916974177273193No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT1467270.11883928841599646No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1461520.11837357596471483No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC1460970.11832902955633139No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA1428050.11566272453090688No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1411000.11428178587101964No Hit
CTGGCAATTTTTGACAGTCTCTACGGAGACTG1374880.1113563017422732No Hit
CAGAGTGGCGCAGCGGAAGCGTGCTGGGC1357190.10992352726172158No Hit
CCCGCGAGGGGGGCCCGGGC1346290.1090406984410312No Hit
GCATAATTTGTGGTAGTGGGGG1337330.10831499695172976No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG1319920.10690490064271883No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1315380.1065371902898808No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC1304450.10565193166509679No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT1287660.10429205130735447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)