FastQCFastQC Report
Tue 25 Jul 2023
SRR8731920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8731920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122440661
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGGGGACTAGATCGGAA7287900.5952189362976406No Hit
AGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTG6501510.5309927230791411TruSeq Adapter, Index 12 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTC5744600.46917420676126537TruSeq Adapter, Index 12 (100% over 50bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGGGACTAGATCGGAA3755940.306755939515877No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGGGTAGCAGATCGGAA3688690.30126348305159834No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGGTGACTAGATCGGAA2927010.23905539026778042No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGGGACTAGATCGGAAG2886740.23576645016641978No Hit
GAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCT2588480.21140689529599976TruSeq Adapter, Index 12 (100% over 50bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGGTAGCAGATCGGAA2536170.20713462172504932No Hit
CACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCT2373070.19381388344514083TruSeq Adapter, Index 12 (100% over 50bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGGGACTAGATCGGAA2036220.16630259779469828No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGGGGACTAGATCGGAAG1877390.1533305998731908No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGGTGACTAGATCGGAAG1681240.13731059488481528No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1367180.1116606190160963TruSeq Adapter, Index 12 (100% over 49bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGGGACTAGATCGGAAG1358060.11091576841454655No Hit
GCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGC1327780.1084427337418572TruSeq Adapter, Index 12 (100% over 50bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGGTAGCAGATCGGAAG1321510.10793064895329176No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGGAGACTAGATCGGAA1310390.10702245392157758No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCGGGATCGAGATCGGAA1292330.10554745371719285No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGCGGGGACTAGATCGGAAG1249940.10208536851985797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)