FastQCFastQC Report
Fri 14 Jul 2023
SRR8756003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8756003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37072369
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length18-35
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTATTAGATAAAAAATCAATG13824553.7290711041422795No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA11965483.227600588459831No Hit
ATGTATTTATTAGATAAAAAATCA6997081.8874110796642103No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT4597631.2401770170123199No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC3757141.0134609957081513No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT3544200.9560219903939777No Hit
AAGAGGTGCACAATCGACCGATCCTGA3458070.9327890537559119No Hit
ATGTATTTATTAGATAAAAAATCAAT2969580.8010224542165082No Hit
ACGCGAAACTCAGGTGCTGCAATCT2821280.7610196154445916No Hit
TGATGTATTTATTAGATAAAAAATCAATG2481110.669261249530614No Hit
CAAGAGGTGCACAATCGACCGATCCTGA2396030.6463115427017896No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA2289220.6175003275350437No Hit
ATACGCGAAACTCAGGTGCTGCAATCT2276190.6139855804736946No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC2169250.5851392987591378No Hit
TTTGCTCCACACAATATAGATGGAT2018830.5445646055152289No Hit
TCCAACGGGGCCTTTCCTTCTGGC1877300.5063879246562312No Hit
AAATATAGATGGATACGAATAAGGCGTCC1838970.4960486879055395No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGC1823140.49177866135287984No Hit
AAAATCAGTTATCGTTTATTTGATAGTT1761740.47521646107913956No Hit
AAGCCTTTGTTGTCTGATTTGT1713170.4621150593316548No Hit
TTTGCTCCACACAATATAGATGGATACGAAT1693000.45667434956746356No Hit
TTGGGCCAGCATCAGTTTTGGTGGC1499070.40436315251393834No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1480480.3993486361769867No Hit
TGATGTATTTATTAGATAAAAAATCA1420250.3831020348335441No Hit
TATGTATTTATTAGATAAAAAATCAATG1393270.3758243774494152No Hit
ATGTATTTATTAGATAAAAAATCAATGT1281960.3457993202430629No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1148280.3097401193864897No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC1109470.29927140615157344No Hit
AAATAGAATAGGACGTTTGGTTCTA1105570.2982194097172479No Hit
ATGGAATAATAGAATAGGACGTTTGGTTCTAT1101320.29707300334650855No Hit
TTTGACCTATTCTCAAACTTTAAATATGT1076650.29041845154271095No Hit
TCACGTTCTAGCATTCAAGGTCCCA1043000.28134161051321No Hit
TTGGAAGAGATGTATTTATTAGATA1040840.28075896633419894No Hit
ATGCCGACTAGGGATCGGGTGGTGTT1030920.2780831189935556No Hit
TTAATGACCCACTCGGCACCTTACGAGAAATC1021290.2754854970287979No Hit
ATGGAATAATAGAATAGGACGTTTGGTTCTA1020980.2754018767994028No Hit
AAATAGAATAGGACGTTTGGTTCTAT982520.26502757350090034No Hit
TCGCTTGCTACAATTAACGATCAACTT975250.2630665442502474No Hit
ATGTATTTATTAGATAAAAAATCAA944690.2548232080879428No Hit
ATTCAATGAAGCGGAGCTGGAATT943880.2546047165208137No Hit
TCGGGTCTTGTAATTGGAATGAGTAC938470.25314540864653134No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT903960.2438365889161278No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT864140.23309543557898876No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGTT836230.2255669175066746No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTC832580.22458235674121607No Hit
TTTGACCTCAAATCAGGTAGGAGT814610.21973508086305465No Hit
TTTGGAAGAGATGTATTTATTAGATA813080.21932237456958847No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCTG803190.21665461950920914No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTT797560.21513596824632386No Hit
TTGATGTATTTATTAGATAAAAAATCAATG796730.21491208182568533No Hit
ATTCAATGAAGCGGAGCTGGAAT775570.2092043268127807No Hit
TATGTATTTATTAGATAAAAAATCA770040.20771264981744222No Hit
TCTATGCCGACTAGGGATCGGGTGGTGT746010.20123073332594418No Hit
AGTTGAACTTTGGGCCCGGTTGG741030.19988741480211314No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC740550.19975793831788846No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA720680.19439815135633765No Hit
ATGCATGTCTAAGTATAAGCAAT706800.19065412302084067No Hit
ATGCCGACTAGGGATCGGGTGGTGTTT704190.18995009463786897No Hit
AAAGAGGTGCACAATCGACCGATCCTGA703670.18980982844662556No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG702480.18948883466281854No Hit
ATGTATTTATTAGATAAAAAATC696860.18797288082668792No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC683910.18447971317937625No Hit
TAAGAGGTGCACAATCGACCGATCCTGA678400.18299343103754712No Hit
AAATATAGATGGATACGAATAAGGCGTC669550.1806062083596546No Hit
TCCACGTTCTAGCATTCAAGGTCCCA653690.17632808952673082No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC648260.17486338679893912No Hit
AAATGACCCACTCGGCACCTTACGAGAAATC637510.17196365303765723No Hit
AATGTATTTATTAGATAAAAAATCAATG630060.16995407010542002No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC629890.16990821385059043No Hit
TTTGGAATGAGTACAATGTAAATACCTT626960.16911786781146895No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTC618380.16680347565595283No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGT617830.16665511718444537No Hit
TTGACCTATTCTCAAACTTTAAATATGT616510.1662990568528275No Hit
TTCCAACGGGGCCTTTCCTTCTGGC609160.16431644818813712No Hit
TTTTCCACGTTCTAGCATTCAAGGTC595330.1605859069864135No Hit
TCTATGCCGACTAGGGATCGGGTGGTG591070.1594368031889195No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT577390.1557467233885161No Hit
ACTTGCGTGCCTTGTTGTAGACGGCCTTGG541970.14619243782343663No Hit
TTGGAATGAGTACAATGTAAATACCTT537480.14498129321058495No Hit
TTTGACCTCAAATCAGGTAGGAGTACCC534130.1440776552477669No Hit
TTTGTGGCGTCGCTGAACCATAGC523940.14132897738474712No Hit
TCAATATAGATGGATACGAATAAGGCGTCC522730.14100258874743074No Hit
CTACATGGTATAACTGTGGTAATTCT519640.14016908388023436No Hit
TGATGTATTTATTAGATAAAAAATCAAT517320.1395432808731484No Hit
ATGCATGTCTAAGTATAAGCAATT513890.13861806349629288No Hit
AAGCCATGCATGTCTAAGTATAAGCAAT512140.13814601381422373No Hit
TTACGCGAAACTCAGGTGCTGCAATCT505910.1364655169460576No Hit
TTTGCTCGAATATATTAGCATGGAAT492240.1327781345724089No Hit
CGTGAATCATCGAATCTTTGAACGCAC487470.13149146201042614No Hit
AAATTCTAGAGCTAATACATGCTT484350.13064986486296573No Hit
TCCAACGGGGCCTTTCCTTCTGGCT484340.13064716743621105No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT480950.12973273976637426No Hit
AAGAGGTGCACAATCGACCGATCCT480740.12967609380452597No Hit
GATGTATTTATTAGATAAAAAATCAATG476590.1285566617013334No Hit
GTTTTCATGCCAGGTCGTACTGAT475850.12835705212148701No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA474010.12786072559862577No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTT467420.1260831213672911No Hit
ACGTTCTAGCATTCAAGGTCCCA467150.12601029084491472No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG463090.1249151355825143No Hit
TTGATGTATTTATTAGATAAAAAATCA457670.1234531302814773No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGCA457620.12343964314770388No Hit
TATAGATGGATACGAATAAGGCGTCC446390.12041043290219731No Hit
TCCGATATAGTGTAACGGCTATCACATCACG444790.11997884462144838No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT442030.11923435483715647No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTG436420.11772109842778054No Hit
TCGTGAATCATCGAATCTTTGAACGCAC434150.11710878255446798No Hit
TGATGTATTTATTAGATAAAAAATC431850.1164883744008914No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGC428810.11566835666746843No Hit
ACTATGCCGACTAGGGATCGGGTGGTG427150.11522058382619142No Hit
TTTGACCTATTCTCAAACTTTAAAT424800.11458668853884142No Hit
AGTTTTCATGCCAGGTCGTACTGATAACCGC421550.11371002484357015No Hit
AGAATCCATGCTAGAACGCGGTGATTT415190.11199446142759314No Hit
ACTTATTCAATGAAGCGGAGCTGGAATT414860.11190544634468867No Hit
TGTCGCTTGCTACAATTAACGATCAACTT414250.11174090331265314No Hit
TCGGGTCTTGTAATTGGAATGAGT414040.11168425735080487No Hit
TTTGCTCGAATATATTAGCATGGAATAAT407880.11002264246992148No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT405350.10934019350098721No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT398460.10748166646701214No Hit
AGGACTTTTACTTTGAAAAAATTAGAGTGTTC394950.10653486967611915No Hit
TTTGCTCCACACAATATAGATGGATAC394890.10651868511559108No Hit
ACTTATTCAATGAAGCGGAGCTGGAAT393840.1062354553063496No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTTT390570.10535339675756897No Hit
TATACGCGAAACTCAGGTGCTGCAATCT385720.10404514478154876No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA384130.1036162539275545No Hit
ATATAGATGGATACGAATAAGGCGTCC383110.10334111639857706No Hit
TACGCGAAACTCAGGTGCTGCAATCT381630.1029418972388843No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT380010.102504914104626No Hit
TGGTTGCGGCCATATCTACCAGAAAGC379750.10243478100900431No Hit
CCACGTTCTAGCATTCAAGGTCCCA378120.10199510044799133No Hit
ATGTACACGGAGTCGACCCAACGCGA375320.10123982095668072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)