FastQCFastQC Report
Fri 14 Jul 2023
SRR8756004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8756004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38961594
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length18-36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCA28105327.213596035110884No Hit
ATGTATTTATTAGATAAAAAATCAATG22782475.847417331026036No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC6815141.7491943476439902No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA5450041.3988236723579637No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT5004001.2843417032680953No Hit
ATGTATTTATTAGATAAAAAATCAAT4884631.253703839735099No Hit
TGATGTATTTATTAGATAAAAAATCAATG4622591.1864478645303886No Hit
AAGAGGTGCACAATCGACCGATCCTGA3961621.0168013146484716No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC3924211.0071995514351904No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT3668670.9416118857970749No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3091170.7933889973803433No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT2761430.7087569363820176No Hit
TATGTATTTATTAGATAAAAAATCAATG2454610.630007591578517No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT2311700.5933278807843436No Hit
ATGTATTTATTAGATAAAAAATCAATGT2273660.5835644198746078No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG2108310.5411251911305272No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA2103380.5398598424900173No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG1885630.4839714720090764No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT1884670.4837250755192408No Hit
ATGTACACGGAGTCGACCCAACGCGA1648850.42319880444316527No Hit
TTGATGTATTTATTAGATAAAAAATCAATG1492910.38317477462549404No Hit
TCTATGCCGACTAGGGATCGGGTGGTGT1478100.3793735954437593No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1450540.3722999628813955No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1383990.3552190395495626No Hit
ACGCGAAACTCAGGTGCTGCAATCT1311190.3365339724036958No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT1305700.3351248924774484No Hit
AAATATAGATGGATACGAATAAGGCGTCC1261060.32366745570009275No Hit
TCAATATAGATGGATACGAATAAGGCGTCC1208550.3101900810321056No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA1166880.29949493339517885No Hit
TGATGTATTTATTAGATAAAAAATCAAT1154080.29620964686403745No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1149910.29513936211131403No Hit
TCCACGTTCTAGCATTCAAGGTCCCA1109200.2846906109642229No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1096800.2815079896371796No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC1095880.2812718596677538No Hit
ACTATGCCGACTAGGGATCGGGTGGTG1095300.2811229951218115No Hit
ACGTTCTAGCATTCAAGGTCCCA1093350.2806225022518329No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1090480.27988587941242854No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG1047880.26895203517597355No Hit
AATGTATTTATTAGATAAAAAATCAATG1019160.261580673521725No Hit
TTACGCGAAACTCAGGTGCTGCAATCT1013000.2599996293786132No Hit
TCCGATATAGTGTAACGGCTATCACATCACG990120.2541271797041979No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA989070.25385768354344024No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT971770.24941741346619445No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGCA944480.24241307991659683No Hit
GATGTATTTATTAGATAAAAAATCAATG940220.24131969549295135No Hit
TTTGGAAGAGATGTATTTATTAGATA937060.24050864038057582No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTT912040.23408693186423532No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT892310.22902297067209312No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT874270.22439276996726573No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA873350.22415663999783994No Hit
AGAGATGTATTTATTAGATAAAAAATCA800290.20540484046930935No Hit
AAAGAGGTGCACAATCGACCGATCCTGA797600.20471441697174916No Hit
TAAGAGGTGCACAATCGACCGATCCTGA770150.1976690173405123No Hit
AAATACTGAATATCTAAATATTATTATTACTT756990.19429133212568253No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT756230.19409626823789602No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT726390.1864374440121726No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG710260.18229746965691393No Hit
TACGCGAAACTCAGGTGCTGCAATCT701880.1801466336310573No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC674410.17309610073961554No Hit
ATTTATTAGATAAAAAATCAATG666970.1711865279433896No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC632690.1623881199521765No Hit
TGATGTATTTATTAGATAAAAAATCAATGT563400.14460393997227117No Hit
TTTGACCTCAAATCAGGTAGGAGTACCCGCTGAA553330.14201934345909975No Hit
TATGTATTTATTAGATAAAAAATCAAT538840.13830029644064357No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA530640.1361956597566311No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC512820.13162192491405766No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC504740.12954808779127466No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT498650.12798501005888005No Hit
TGATGTATTTATTAGATAAAAAATCA498140.12785411192365487No Hit
TTGCTGGCGAATTGCAATGTCATTTTG494330.1268762258546198No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA462220.11863477659563929No Hit
ATATAGATGGATACGAATAAGGCGTCC450330.11558305340382122No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCC440560.11307545579372343No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT439970.11292402461767863No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC438160.11245946456913442No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGA432810.1110863174643214No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT431710.11080398815305142No Hit
TCAATATAGATGGATACGAATAAGGCGTC424680.1089996471910261No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA424130.10885848253539114No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA421990.10830922369346593No Hit
AAATATAGATGGATACGAATAAGGCGTC402170.10322216283040166No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTT396190.10168731802913401No Hit
TCTTTGAACGCACATTGCGCCCCTTGGT395920.10161801901636776No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT393660.1010379606132131No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT391010.10035780363606274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)