FastQCFastQC Report
Fri 14 Jul 2023
SRR8756005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8756005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24462144
Total Bases721.7 Mbp
Sequences flagged as poor quality0
Sequence length18-36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCA9524683.8936407209441657No Hit
ATGTATTTATTAGATAAAAAATCAATG8063153.2961746934365195No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG6566262.684253677846063No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT5251782.1469009421251055No Hit
TGATGTATTTATTAGATAAAAAATCAATG5119072.0926497693742623No Hit
AGAGATGTATTTATTAGATAAAAAATCA3663131.4974689054238255No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA2567451.049560496414378No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG2494761.0198451942724236No Hit
TTTGGAAGAGATGTATTTATTAGATA2462521.0066656463145667No Hit
ATGTATTTATTAGATAAAAAATCAAT2200500.8995532035131507No Hit
TGATGTATTTATTAGATAAAAAATCA2022840.826926699474911No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1994600.815382331164431No Hit
ATGTACACGGAGTCGACCCAACGCGA1941170.7935404190245958No Hit
TTGATGTATTTATTAGATAAAAAATCAATG1665450.6808274859309144No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1586000.648348730184893No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA1531240.6259631208123049No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT1493470.6105229369919497No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT1457820.5959493983847042No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT1440620.588918125900984No Hit
TGATGTATTTATTAGATAAAAAATCAAT1375780.5624118638170064No Hit
AAGAGGTGCACAATCGACCGATCCTGA1191180.486948323090568No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT1177120.48120066663003863No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1173240.4796145423720832No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT1120180.45792388434963016No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT1118900.45740062686246963No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1049170.4288953576595739No Hit
GATGTATTTATTAGATAAAAAATCAATG991710.4054060020250065No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC981680.40130578905920916No Hit
TCTATGCCGACTAGGGATCGGGTGGTGT928920.37973776951030946No Hit
ATGTATTTATTAGATAAAAAATCAATGT878990.3593266395619288No Hit
TATGTATTTATTAGATAAAAAATCAATG858890.3511098618338605No Hit
TCCGATATAGTGTAACGGCTATCACATCACG800280.32715039205067226No Hit
TTTGGAAGAGATGTATTTATTAGATAA789310.32266591186774146No Hit
TTACGCGAAACTCAGGTGCTGCAATCT745140.30460944061158335No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG685660.28029431925509063No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT685430.2802002964253665No Hit
TGATGTATTTATTAGATAAAAAATCAATGT681030.278401598813252No Hit
TTGATGTATTTATTAGATAAAAAATCA652760.2668449666554166No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA645170.2637422132745192No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC609820.24929131314082692No Hit
AGAGATGTATTTATTAGATAAAAAATC607290.2482570620138611No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA605840.24766430939168702No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT604700.24719828319218462No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTT600150.24533826634329353No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT594710.2431144220228611No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT571680.23369987520308932No Hit
ACTATGCCGACTAGGGATCGGGTGGTG563680.23042951590833577No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT553370.2262148403672221No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG545660.22306303159690336No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT533830.21822698778978653No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA528800.21617074938321024No Hit
TACGCGAAACTCAGGTGCTGCAATCT524750.21451512999024125No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT495640.20261511010645672No Hit
TTGGAAGAGATGTATTTATTAGATAA495620.20260693420821987No Hit
AAATACTGAATATCTAAATATTATTATTACTT492310.20125382305001557No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC486610.19892369205250365No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC474130.1938219315526881No Hit
AGATGTATTTATTAGATAAAAAATCAATG473010.1933640812514226No Hit
TCAATATAGATGGATACGAATAAGGCGTCC465100.19013051349873503No Hit
ACGCGAAACTCAGGTGCTGCAATCT457800.1871463106422724No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGA452060.18479982784828672No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTT451640.18462813398531217No Hit
TTGATGTATTTATTAGATAAAAAATCAAT441130.18033169946182967No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGA440330.1800046635323543No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC417710.17075772262643865No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC410510.16781439926116043No Hit
AGAGATGTATTTATTAGATAAAAAATCAA399370.1632604239432161No Hit
ATACGCGAAACTCAGGTGCTGCAATCT383330.15670335355723522No Hit
TTTTGGAAGAGATGTATTTATTAGATA377420.15428737562823602No Hit
ATTTATTAGATAAAAAATCAATG370490.15145442688915575No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT355450.14530615141501907No Hit
AATGTATTTATTAGATAAAAAATCAATG353640.14456623262458107No Hit
ACGTTCTAGCATTCAAGGTCCCA348930.1426408085897949No Hit
GATGTATTTATTAGATAAAAAATCA348860.1426121929459658No Hit
TAGAGATGTATTTATTAGATAAAAAATCA346030.14145530334544676No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT343710.1405068991499682No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGCA329700.13477968243503105No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCC316890.1295430196143069No Hit
TCTATGCCGACTAGGGATCGGGTGGTG312660.12781381713720597No Hit
TGATGTATTTATTAGATAAAAAATCAA306660.12536104766614078No Hit
TTGGAAGAGATGTATTTATTAGATA300820.12297368538097068No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT299740.12253218687617896No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG295060.12061902668874813No Hit
GAGATGTATTTATTAGATAAAAAATCAATG287920.11770023101818057No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG277850.11358366625590953No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA276600.1130726726161043No Hit
CGATGTATTTATTAGATAAAAAATCAATG267850.10949571713746759No Hit
GATGTATTTATTAGATAAAAAATCAAT267030.10916050530975535No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC266800.10906648248003119No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAG263440.10769293157623469No Hit
TTGGCGTCGCTGAACCATAGCAGGCTAGCA263270.10762343644122117No Hit
TTTGGAAGAGATGTATTTATTAGATAAA262030.10711653075053437No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT258440.10564895701701371No Hit
AAATATAGATGGATACGAATAAGGCGTCC256420.10482319129508845No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA256420.10482319129508845No Hit
TCCACGTTCTAGCATTCAAGGTCCCA255790.1045656505006266No Hit
ATGTATTTATTAGATAAAAAATCAA253810.1037562365751751No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA253240.10352322347542392No Hit
TTTGACCTCAAATCAGGTAGGAGTACCCGCTGAA252640.10327794652831739No Hit
ATTAGATAAAAAATCAATG246310.10069027473634364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)