FastQCFastQC Report
Fri 14 Jul 2023
SRR8756006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8756006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24831314
Total Bases737.3 Mbp
Sequences flagged as poor quality0
Sequence length18-36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCA14567265.866487774267604No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG8004393.223506416132469No Hit
ATGTATTTATTAGATAAAAAATCAATG7920383.189674134844415No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT6218822.5044264673226717No Hit
TGATGTATTTATTAGATAAAAAATCAATG4968162.000764035282225No Hit
AGAGATGTATTTATTAGATAAAAAATCA4586201.8469421312138374No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA3693101.4872753008560078No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG3237051.3036160712236171No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT2715441.0935546946891332No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA2545371.0250645616256957No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC2209910.8899690125137961No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT2122620.8548158184460154No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT1822870.7341013045060765No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT1760490.7089797986526206No Hit
TTGATGTATTTATTAGATAAAAAATCAATG1622520.6534168912688229No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTT1615110.6504327559951116No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGT1540380.620337691352137No Hit
TGATGTATTTATTAGATAAAAAATCAAT1521850.6128753395813045No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT1436060.5783262214798621No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT1353740.5451745324472157No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1321300.5321103828818724No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT1304170.5252118353462889No Hit
ATGTATTTATTAGATAAAAAATCAAT1299490.5233271183313134No Hit
AAGAGGTGCACAATCGACCGATCCTGA1208750.48678454954095457No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1173520.47259681867822223No Hit
TTTGGAAGAGATGTATTTATTAGATAA1135160.4571485826323971No Hit
GATGTATTTATTAGATAAAAAATCAATG1007780.40585045157094785No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT996750.4014084796318068No Hit
TCTATGCCGACTAGGGATCGGGTGGTGT933480.3759285553716569No Hit
TATGTATTTATTAGATAAAAAATCAATG879120.3540368423515566No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA878140.35364217938688225No Hit
ACGTTCTAGCATTCAAGGTCCCA864180.348020245726827No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG847360.3412465405576201No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT804310.3239095603237106No Hit
ATGTATTTATTAGATAAAAAATCAATGT778850.31365637758839504No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC761350.3066088246477814No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT754750.3039508903958929No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACC721090.2903954257112612No Hit
AGAGATGTATTTATTAGATAAAAAATCAA721020.2903672354994987No Hit
AGAGATGTATTTATTAGATAAAAAATC720110.2900007627465868No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAG684940.2758371949225079No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGA660600.2660350555753916No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT654140.26343350174702795No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCC628750.2532085092234748No Hit
ACTATGCCGACTAGGGATCGGGTGGTG596580.24025309333207256No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA596460.24020476725476547No Hit
AAATACTGAATATCTAAATATTATTATTACTT581740.2342767684384322No Hit
TGATGTATTTATTAGATAAAAAATCAATGT570710.22983479649929117No Hit
TTTGGAAGAGATGTATTTATTAGATA567810.2286669162977038No Hit
ATTTATTAGATAAAAAATCAATG554030.22311747175360916No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT552410.2224650697099638No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA511730.20608252950286882No Hit
AGATGTATTTATTAGATAAAAAATCAATG507260.2042823831231807No Hit
TTGATGTATTTATTAGATAAAAAATCAAT487990.19652202054228785No Hit
TCAATATAGATGGATACGAATAAGGCGTCC480110.19334860813245727No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTT452050.18204836038882194No Hit
TAGAGATGTATTTATTAGATAAAAAATCA441140.17765471452698797No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA435440.1753592258549024No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT432570.17420342717264178No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA419420.16890769453440926No Hit
TTTGGAAGAGATGTATTTATTAGATAAA407960.16429255415158459No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTT406890.1638616466289299No Hit
TCCGATATAGTGTAACGGCTATCACATCACG397660.16014456584939482No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCC396500.15967741376875988No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG394460.15885587045453978No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC361690.14565882417660217No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGA350650.1412128250643522No Hit
AATGTATTTATTAGATAAAAAATCAATG349090.14058458605936036No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT346880.13969458080228858No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGCA344170.13860321688977073No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT338200.13619899454374423No Hit
TTTTGGAAGAGATGTATTTATTAGATA336640.1355707555387524No Hit
GAGATGTATTTATTAGATAAAAAATCAATG321020.12928031114261615No Hit
TTACGCGAAACTCAGGTGCTGCAATCT320400.12903062640986296No Hit
TCTATGCCGACTAGGGATCGGGTGGTGTT311890.1256035020941703No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTT297110.11965134023918346No Hit
ATTAGATAAAAAATCAATG295270.11891034038714182No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGTT294540.11861635675019051No Hit
GATGTATTTATTAGATAAAAAATCAAT293890.11835459049811058No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACG285320.11490330314376436No Hit
TCCACGTTCTAGCATTCAAGGTCCCA284820.11470194448831826No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC279610.11260378729856986No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA273690.110219700818088No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT273550.11016332039456309No Hit
CGATGTATTTATTAGATAAAAAATCAATG267530.107738962182992No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG263480.10610795707387859No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT256950.10347821303375246No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTT254460.10247544692963087No Hit
TGATGTATTTATTAGATAAAAAATCAA251690.10135991997845945No Hit
AAATATAGATGGATACGAATAAGGCGTCC250170.1007477896659033No Hit

[OK]Adapter Content

Adapter graph