FastQCFastQC Report
Fri 14 Jul 2023
SRR8756015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8756015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100150100
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length18-36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCAT55833665.574997928109907No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAT51161425.1084741802554365No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT29295322.9251413628144154No Hit
AAGAGGTGCACAATCGACCGATCCTGA18784101.8755947323068074No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA9551020.9536705405186814No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATA7309640.729868467430387No Hit
ATGTATTTATTAGATAAAAAATCAATG7132070.7121380807408081No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA5888730.5879904263700186No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA4786560.4779386141401756No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT4497800.4491058920560239No Hit
AAGAGGTGCACAATCGACCGATCCTG4467410.44607144675841565No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG4255500.4249122067776268No Hit
ATATTAGCATGGAATAATAGAAT4206150.41998460311073077No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3758970.37533362423003075No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC2934370.2929972111860098No Hit
TAAGAGGTGCACAATCGACCGATCCTGA2897850.28935068462238184No Hit
TTTGCTCGAATATATTAGCATG2882660.28783396122420246No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2637980.26340263264839475No Hit
AGGCGGCCAGGCCGGGAACCATCCCTGTGAGGC2328400.23249103096252524No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAAT2142650.21394387025075362No Hit
AAATACTGAATATCTAAATATTATTATTACTT2100880.20977313053107288No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT2085040.20819150455166796No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1994730.1991740397663108No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1985520.19825442011540678No Hit
ATTAGCATGGAATAATAGAAT1869030.18662287905853314No Hit
TAAGGCGGCAGACTTAAGATCTGTTG1639750.16372924240714687No Hit
TTTCTTTACTTATTCAATGAAGCGGAG1609630.16072175664327842No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1460920.14587304456011527No Hit
TATTAGCATGGAATAATAGAAT1452560.14503829751542932No Hit
ATAAATCATTTGTATACGACTTAGAT1426460.14243220925390987No Hit
ATATAGATGGATACGAATAAGGCGTC1417780.141565510169236No Hit
AGAGGTGCACAATCGACCGATCCTGA1339000.13369931732469564No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA1265530.1263633286437058No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGA1227600.12257601340388079No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1178400.11766338725572914No Hit
ATATTAGCATGGAATAATAGAATA1155740.11540078342408046No Hit
TTGGGTAAAACCATACTGCAGACATCT1120810.11191301855914274No Hit
AAATATAGATGGATACGAATAAGGCGTC1097060.10954157809128498No Hit
CAAGAGGTGCACAATCGACCGATCCTG1078950.10773329232821535No Hit
AGGCGGCCAGGCCGGGAACCATCCCTGTGAGGCG1074200.1072590042346438No Hit
ATATAGATGGATACGAATAAGGCGTCC1025140.10236035710398692No Hit
GTTTAGTGGTAAAATCCAACGT1024670.10231342754525458No Hit
TCTGACTTCTAATCAGAAGATTATGGGT1004670.10031642504600595No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1003790.100228556936039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)