FastQCFastQC Report
Fri 14 Jul 2023
SRR8763378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8697683
Total Bases443.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2973703.4189565197995835No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2887873.320275066359627No Hit
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2538642.9187543395177773No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACACTAATCTCGTAT865010.994529232670356TruSeq Adapter, Index 4 (97% over 37bp)
GACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384520.442094750981382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350540.4030268750884575No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTTACACTAATCTCGTATGCCGT193690.22269149151561396TruSeq Adapter, Index 4 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)