FastQCFastQC Report
Fri 14 Jul 2023
SRR8763379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112536
Total Bases105.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTAT33644115.925929782971746TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCCGT702523.3254817906061716TruSeq Adapter, Index 14 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATG282851.3389120942791033TruSeq Adapter, Index 14 (97% over 36bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106520.5042280936277536No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103280.4888910768857903No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAGCTCGTAT83010.3929400493056686TruSeq Adapter, Index 14 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCC81510.3858395785917968TruSeq Adapter, Index 14 (97% over 34bp)
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78490.37154396422120145No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTATG67830.32108328568128547TruSeq Adapter, Index 14 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAACCTCGTAT53450.25301343977096724TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTATGCCGT45050.2132508037732848TruSeq Adapter, Index 14 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTAT35900.16993793241866648TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAGCTCGTATGCCGT26340.12468426573558984TruSeq Adapter, Index 14 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)