FastQCFastQC Report
Fri 14 Jul 2023
SRR8763380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942933
Total Bases99 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTAT27753814.284486392479822TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCCGT560302.883784464003648TruSeq Adapter, Index 14 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATG318851.6410756315323276TruSeq Adapter, Index 14 (97% over 36bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105510.5430449737587452No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99490.5120608893873335No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75110.3865804945409852No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCC67970.34983192935628765TruSeq Adapter, Index 14 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAGCTCGTAT63540.3270313489965943TruSeq Adapter, Index 14 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAACCTCGTAT42690.2197193624278346TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTATGCCGT35940.18497807181204912TruSeq Adapter, Index 14 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTAT30430.1566188849538301TruSeq Adapter, Index 14 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)