FastQCFastQC Report
Fri 14 Jul 2023
SRR8763381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151001
Total Bases109.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTAT23719211.027052056228705TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCCGT487082.264434093707999TruSeq Adapter, Index 14 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATG264371.229055681517582TruSeq Adapter, Index 14 (97% over 36bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116100.5397487030456982No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107720.5007900972616935No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85540.3976753148882776No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCC56070.2606693348817597TruSeq Adapter, Index 14 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAGCTCGTAT45790.21287763232095197TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTATGCCGT31970.1486284757654692TruSeq Adapter, Index 14 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAACCTCGTAT31770.1476986761047531TruSeq Adapter, Index 14 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTAT24770.11515568797968946TruSeq Adapter, Index 14 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)