FastQCFastQC Report
Fri 14 Jul 2023
SRR8763382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9729242
Total Bases496.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTAT155071615.938713416728662TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCCGT3144523.2320297922489747TruSeq Adapter, Index 14 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATG1804621.8548413124064547TruSeq Adapter, Index 14 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCC373370.3837606259562667TruSeq Adapter, Index 14 (97% over 34bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371680.3820235944382923No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357920.367880663262359No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254340.2614181043086399No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAGCTCGTAT166470.1711027436669784TruSeq Adapter, Index 14 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAACCTCGTAT143850.1478532448879368TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTATGCCGT130130.13375142688402653TruSeq Adapter, Index 14 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTAT123420.12685469227715787TruSeq Adapter, Index 14 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)