FastQCFastQC Report
Fri 14 Jul 2023
SRR8763383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450482
Total Bases124.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTAT37607015.346776674956192TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCCGT776683.1694988985840333TruSeq Adapter, Index 14 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATG399161.6289040278606413TruSeq Adapter, Index 14 (97% over 36bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118950.485414706168011No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116860.4768857718603932No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAGCTCGTAT105960.4324047269067881TruSeq Adapter, Index 14 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAATCTCGTATGCC90530.36943752290365733TruSeq Adapter, Index 14 (97% over 34bp)
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87260.35609320941757583No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAACCTCGTAT61510.2510118417519492TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTATGCCGT49250.20098086825367417TruSeq Adapter, Index 14 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTAT45440.1854329066689737TruSeq Adapter, Index 14 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAGCTCGTATGCCGT34580.14111509490785895TruSeq Adapter, Index 14 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTATCAAACTCGTATG34240.13972761277169146TruSeq Adapter, Index 14 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)