FastQCFastQC Report
Fri 14 Jul 2023
SRR8763418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384067
Total Bases69.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTAT517293.737463576546511TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCCGT355962.571840814064637TruSeq Adapter, Index 7 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATG130490.9428011794226724TruSeq Adapter, Index 7 (97% over 35bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80430.5811134865580929No Hit
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78610.567963834120747No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57290.4139250484261238No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCC35680.2577909884420335TruSeq Adapter, Index 7 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTATGCCGT33420.2414622991517029TruSeq Adapter, Index 7 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTAT32020.2313471818922061TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAACTCGTATGCCGT29220.21111694737321243TruSeq Adapter, Index 7 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAACTCGTATG27320.19738928823532387TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTATG21350.15425553820732665TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGACCTCGTAT16120.11646835015934921TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGACCTCGTATGCCGT15440.11155529320473648TruSeq Adapter, Index 7 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)