FastQCFastQC Report
Fri 14 Jul 2023
SRR8763419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415494
Total Bases21.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTAT105922.5492546222087444TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCCGT59431.430345564556889TruSeq Adapter, Index 7 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATG33450.8050657771231354TruSeq Adapter, Index 7 (97% over 35bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.47413440386624117No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19000.45728698850043564No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.32852459963320774No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTAT7010.1687148310204239TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCC6560.15788434971383464TruSeq Adapter, Index 7 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAACTCGTATGCCGT5080.12226410008327436TruSeq Adapter, Index 7 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)