FastQCFastQC Report
Fri 14 Jul 2023
SRR8763420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492702
Total Bases25.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTAT86501.7556251040182504TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCCGT49060.9957337295160157TruSeq Adapter, Index 7 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATG25310.5136979350601377TruSeq Adapter, Index 7 (97% over 35bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20770.42155298740415104No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19360.3929352833964546No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14380.29185998839054844No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCC5040.1022930696445316TruSeq Adapter, Index 7 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)