FastQCFastQC Report
Fri 14 Jul 2023
SRR8763421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377780
Total Bases70.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTAT481673.4959862967962954TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCCGT322392.3399236452844434TruSeq Adapter, Index 7 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATG145301.054595073233753TruSeq Adapter, Index 7 (97% over 35bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76730.5569103920800128No Hit
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74760.5426120280451161No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56900.41298320486579865No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTATGCCGT35910.2606366763924574TruSeq Adapter, Index 7 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTAT34130.24771734239138324TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCC32490.23581413578365198TruSeq Adapter, Index 7 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAACTCGTATGCCGT31070.22550770079403099TruSeq Adapter, Index 7 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTATG19720.14312880140515902TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAACTCGTATG16950.1230239951225885TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGACCTCGTAT14080.10219338355905877TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGACCTCGTATGCCGT14030.10183048091857916TruSeq Adapter, Index 7 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)