FastQCFastQC Report
Fri 14 Jul 2023
SRR8763422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324380
Total Bases67.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTAT313182.36472915628445TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCCGT185801.4029206119089688TruSeq Adapter, Index 7 (96% over 31bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67350.5085398450595751No Hit
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65070.4913242422869569No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATG59770.4513055165435902TruSeq Adapter, Index 7 (97% over 35bp)
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48390.36537851673990845No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTAT39750.30014044307525034TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTATGCCGT37570.28367991059967684TruSeq Adapter, Index 7 (96% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAACTCGTATGCCGT36770.2776393482233196TruSeq Adapter, Index 7 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGATCTCGTATGCC21700.1638502544586901TruSeq Adapter, Index 7 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAACTCGTATG21450.16196257871607847TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGAGCTCGTATG20980.1584137483199686TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTAAGGACCTCGTAT14820.11190141802201783TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)