FastQCFastQC Report
Fri 14 Jul 2023
SRR8763545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925481
Total Bases98.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1264226.5657360420591No Hit
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT952994.949360705195222No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509412.645624651710404No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAACTATCTCGTAT197941.0280028730483448TruSeq Adapter, Index 12 (97% over 39bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA49520.25718249102432067No Hit
GACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49000.2544818671282656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41310.21454379451160516No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAACTATCTCGTATG38150.19813231083557822TruSeq Adapter, Index 12 (97% over 38bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA31630.16427064198504165No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCTTGAACTATCTCGTATGCCGT30860.1602716412158832TruSeq Adapter, Index 12 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)