FastQCFastQC Report
Fri 14 Jul 2023
SRR8763548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674987
Total Bases85.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT965185.762313379148614No Hit
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT729034.352451690669838No Hit
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399072.382525953932777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80620.48131716843175504No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAACTATCTCGTAT79970.4774365412985294TruSeq Adapter, Index 12 (97% over 39bp)
GACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37830.22585249915372477No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCTTGAACTATCTCGTATGCCGT28000.1671654765081759TruSeq Adapter, Index 12 (97% over 34bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA19330.11540388074653712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)