FastQCFastQC Report
Fri 14 Jul 2023
SRR8763549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761318
Total Bases238 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTAT68651714.418633664040085TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATG490861.0309330315681497TruSeq Adapter, Index 18 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCCGT408280.8574936603688306TruSeq Adapter, Index 18 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAGCTCGTAT196200.41207077536094TruSeq Adapter, Index 18 (97% over 37bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154290.3240489293090695No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTATG149080.31310658099290994TruSeq Adapter, Index 18 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAACCTCGTAT143680.30176518350591164TruSeq Adapter, Index 18 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCC117180.24610832546786415TruSeq Adapter, Index 18 (97% over 34bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108480.2278360739610335No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTAT105260.22107324064471223TruSeq Adapter, Index 18 (97% over 37bp)
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64020.1344585679847471No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)