FastQCFastQC Report
Fri 14 Jul 2023
SRR8763550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10328928
Total Bases526.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTAT192574018.644141967104428TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATG1807551.749987994881947TruSeq Adapter, Index 18 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCCGT1131371.0953411622193514TruSeq Adapter, Index 18 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAGCTCGTAT495920.4801272697418357TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAACCTCGTAT380420.368305404007076TruSeq Adapter, Index 18 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCC329960.31945231876918884TruSeq Adapter, Index 18 (97% over 34bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321500.31126173016212333No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTAT293630.2842792591835281TruSeq Adapter, Index 18 (97% over 37bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238100.23051762970949163No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTATG152620.1477597675189526TruSeq Adapter, Index 18 (97% over 36bp)
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139740.13528993521883392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)