FastQCFastQC Report
Fri 14 Jul 2023
SRR8763551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11160494
Total Bases569.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTAT163281414.630302207052843TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATG1479041.3252459971753938TruSeq Adapter, Index 18 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCCGT972360.8712517564186675TruSeq Adapter, Index 18 (96% over 32bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338100.30294357937919236No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAGCTCGTAT334980.3001480042012477TruSeq Adapter, Index 18 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCC276440.24769512890737636TruSeq Adapter, Index 18 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAACCTCGTAT261630.23442510698899172TruSeq Adapter, Index 18 (97% over 37bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256620.22993605838594597No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTAT227990.20428307205756302TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTATG151700.13592588285070534TruSeq Adapter, Index 18 (97% over 36bp)
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145480.13035265284852088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)