FastQCFastQC Report
Fri 14 Jul 2023
SRR8763553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4511897
Total Bases230.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTAT67855415.039217428943969TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATG592291.312729435091271TruSeq Adapter, Index 18 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCCGT396770.8793862093926347TruSeq Adapter, Index 18 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAGCTCGTAT218840.48502880274084276TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAACCTCGTAT144210.3196216580298708TruSeq Adapter, Index 18 (97% over 37bp)
CAGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140770.31199737050734977No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTAT117570.2605777569833709TruSeq Adapter, Index 18 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAATCTCGTATGCC112040.24832127151838795TruSeq Adapter, Index 18 (97% over 34bp)
AGGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97340.215740740535522No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTACAAACTCGTATG75900.1682219252788794TruSeq Adapter, Index 18 (97% over 36bp)
GGACATTCGTCGCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58430.12950206975026246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)