FastQCFastQC Report
Fri 14 Jul 2023
SRR8763609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5672366
Total Bases283.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGGATAATCTCGTATG1547232.7276624956852222TruSeq Adapter, Index 12 (97% over 36bp)
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA179150.31582940875112786No Hit
CTCGGTTGATTTCCCTTCCTTTAGCTACTAAGATGTTTCAGTTCGCTAAG166560.29363408496560345No Hit
CCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGA153300.2702575962129383No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTGGATAATCTCGTATGCC148320.261478190934788TruSeq Adapter, Index 12 (97% over 34bp)
GGGGTCTCGGTTGATTTCCCTTCCTTTAGCTACTAAGATGTTTCAGTTCG139840.24652852090291774No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC131790.23233691196936163No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGGATAATCTCGTAT129060.22752410546146TruSeq Adapter, Index 12 (97% over 37bp)
GGTTGATTTCCCTTCCTTTAGCTACTAAGATGTTTCAGTTCGCTAAGTTT125790.22175931524869869No Hit
GTCTCGGTTGATTTCCCTTCCTTTAGCTACTAAGATGTTTCAGTTCGCTA124580.21962616657669834No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGTTGGATAATCTCGTATGCCGT123830.21830396698661547TruSeq Adapter, Index 12 (96% over 32bp)
GCCCGAGGTAGAGCACTCAATGGGCTAGGGTGGCCTCATTTCGCCTTACC118890.2095950790199363No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCA118080.2081671034626468No Hit
GCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGG112790.19884118902059567No Hit
CCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAG106950.18854566154581703No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGGATAAACTCGTATG104130.18357419108710546TruSeq Adapter, Index 12 (97% over 36bp)
GGAGATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAA102910.1814234130872373No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAAGCT102800.18122949048069184No Hit
GGGGAATCTTGGACAATGGGCGAAAGCCCGATCCAGCAATATCGCGTGAG96740.17054611779282225No Hit
GGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGAT92870.16372356790799464No Hit
GCGGAGCTTAGAGCCTTTACTTTATGTAAAGCGCACTCACTCTAACGGCG90680.15986274510495269No Hit
CTCGAGTCCTGTCATGATCGCGCACTCGACGAAAGAGCTTTACAAGCGGC87640.15450342943315012No Hit
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC81200.14315014228630524No Hit
CTGGAAGTTTCAACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG80900.14262126245027207No Hit
GGCCCGGGTACATATTCACCGCGGCATGCTGATCCGCGATTACTAGCGAT80790.14242733984372657No Hit
GGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATACCGTCTGTGAT77280.13623944576213876No Hit
GTCGCGAGAGAGCAGAGCGTACCGCCCTGCCATAGTCACGAGTCTGTTTA77090.1359044885326511No Hit
CCCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGT73820.1301396983198898No Hit
GCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGATGAGCCTGCGTAGT71360.125802883664418No Hit
ACCGAACCCACGTATGTGGCAAAATACGGGGATGACTTGTGGCTAGGGGT70280.12389891625469865No Hit
CCGAACCCACGTATGTGGCAAAATACGGGGATGACTTGTGGCTAGGGGTG70230.12381076961535979No Hit
GGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATACCGTCTGTGATCCAT69870.12317611381212003No Hit
GTGGGGATGACGTCAAGTCCGCATGGCCCTTATGGGCTGGGCCACACACG68430.1206374905991609No Hit
CCCTGATACATTTTCGGTGCCATGGAGCTAGACCAGTGAGCTATTACGCT67680.11931529100907803No Hit
CCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTG65950.11626541728795357No Hit
GGCCGAGTCAGAAAGGCTTTGATGACTCAAGGTTCATATTAGGGAAAGGA65210.11496084702573846No Hit
CCTTCCTTTAGCTACTAAGATGTTTCAGTTCGCTAAGTTTGAAAAGTCCA63760.11240459448491158No Hit
GGGGAGATACCGTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCC63380.11173468002593627No Hit
GCTGACATGAGTAACGAAAAATCCTGTGAAAAACACGATCGCCTGCCAGT63350.11168179204233296No Hit
GGTCATTGAGGAATACTTAGGCTTAGAGGGTGGTCCCCCTTTCTCGCGTA63300.11159364540299409No Hit
CGGTCATTGAGGAATACTTAGGCTTAGAGGGTGGTCCCCCTTTCTCGCGT61800.10894924622282835No Hit
GCTCTACTGAAATAGATTTCGCGGAAAACCAGCTATATCCGATCTTGGTT61340.10813829714091086No Hit
GGCCTTTCACCCCTAGCCACAAGTCATCCCCGTATTTTGCCACATACGTG61230.10794437453436538No Hit
GTCTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAAGCTCCGTGG60350.10639299368200147No Hit
CACGCGTTAGCTGGGCCCCTGATCCGCGTAGACCAAGGGCGAACACTCAT60310.10632247637053038No Hit
GTGCGAAGGTAGGCTCAAGCTAAGATTCTGCTCGTGAGCGTAATGGTATA59910.10561730325581954No Hit
GTGAAATCCGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCT59530.1049473887968442No Hit
CCCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGAC58270.10272609348550499No Hit
GCGGAAAACCAGCTATATCCGATCTTGGTTGGCCTTTCACCCCTAGCCAC58140.10249691222322396No Hit
GTCATGATCGCGCACTCGACGAAAGAGCTTTACAAGCGGCATTGCCCTTC57150.10075160876431458No Hit
GCGGGACACTGCGAGGTGGGTAGTTTATCTGGGGCGGATGCCTCCTAAAG56850.10022272892828143No Hit
CTGGGCTGTTTCCCTCTCGACTTTGGATCTTAGCACCCAAAAAGTCTGTC56820.10016984094467811No Hit
GCCGAGTGACGTGCCAGCGCTACTAATTGAGTGCCAGCACCTAGCTGTGC56740.10002880632173593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)