FastQCFastQC Report
Fri 14 Jul 2023
SRR8763612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9987622
Total Bases499.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAGATATCTCGTATG3680093.684650860835542TruSeq Adapter, Index 7 (97% over 35bp)
GGAACGGTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGT767540.7684912384549596No Hit
GCCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGG597350.5980903161933842No Hit
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA275350.27569125063002986No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAGATATCTCGTAT269970.2703045830128533TruSeq Adapter, Index 7 (97% over 36bp)
GGCGGCCAAAGCCCGGGCTCTCGATACGCCCGTGGAACGCCGTCTCCCCG269340.2696738022324033No Hit
GTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGTG253270.253583886134257No Hit
GTCGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAG243590.24389188938067538No Hit
GCCGGCGATGCGCCCCGGTCGGATGTGGAACGGTGACGAGCCGGTCCGCC223020.22329639627931452No Hit
CGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGGCG216510.2167783282146641No Hit
CGGCGATGCGCCCCGGTCGGATGTGGAACGGTGACGAGCCGGTCCGCCGA213820.21408499440607587No Hit
GTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCG211910.21217262727804476No Hit
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC208930.20918893406258265No Hit
GTTCGACGCCCGGGGAAGGCCCCCGAAGGAACCGTTCCCAGTCCGTCCCC200830.2010788954567964No Hit
GAACGGTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTG195710.1959525500664723No Hit
CCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGGC180450.1806736378289046No Hit
CGGTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGAT176080.17629822193911623No Hit
GTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGATTG173110.1733245411169946No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAGATATCTCGTATGCC169390.16959993079433722TruSeq Adapter, Index 7 (96% over 33bp)
CGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCA167050.16725703075266565No Hit
CCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCGGAGGCGC165920.16612563030519176No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCGGCAGATATCTCGTATGCCGT163170.16337222213656064TruSeq Adapter, Index 7 (96% over 31bp)
GGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCG153650.15384042367642667No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC143960.14413841452950463No Hit
GGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCGGA142010.14218599782811164No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAGATAACTCGTATG141460.1416353161943854TruSeq Adapter, Index 7 (97% over 35bp)
GGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCCAA140490.14066411404035917No Hit
GGTGAATCGGGTTGAAAGTGAAAGCCGCCAAAAACTGGCGGAATGCTCTC131610.1317731087540157No Hit
GCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAA130920.13108225361352283No Hit
CACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCGGAGGCGCG125260.12541523898281293No Hit
GCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGATGAGCCTGCGTAGT120790.12093969915961977No Hit
GGTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGATT120710.12085960001289597No Hit
GGGGAATCTTGGACAATGGGCGAAAGCCCGATCCAGCAATATCGCGTGAG119530.11967813759871969No Hit
GTGAAATCCGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCT113400.11354054048100738No Hit
CGGAAAACCAGCTATATCCGATCTTGGTTGGCCTTTCACCCCTAGCCACA112370.11250926396693828No Hit
CCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTG109690.10982594255169048No Hit
GTCAAATAGGAAGAACTAGAAGATTCCACCTCTGGAAAGCGCCTACACCT104680.1048097334881116No Hit
CTGTGATCCATGGATCTCCGATCGGGAAACCGTATCCAAGCTCCGTGGCT103430.10355818432055197No Hit
GCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGA101110.10123530906556136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)