FastQCFastQC Report
Fri 14 Jul 2023
SRR8763613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300049
Total Bases168.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAGATATCTCGTATG480621.4564026170520499TruSeq Adapter, Index 7 (97% over 35bp)
GGAACGGTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGT182980.5544766153472267No Hit
GCCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGG122800.3721156867670753No Hit
GTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGTG105460.3195710124304215No Hit
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA99010.3000258481010433No Hit
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC85490.2590567594602383No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC60680.18387605759793263No Hit
GTCGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAG57800.17514891445551262No Hit
GCCGGCGATGCGCCCCGGTCGGATGTGGAACGGTGACGAGCCGGTCCGCC56490.17117927642892575No Hit
GGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCCAA56410.17093685578608075No Hit
CGGCGATGCGCCCCGGTCGGATGTGGAACGGTGACGAGCCGGTCCGCCGA54340.16466422165246636No Hit
GGTGAATCGGGTTGAAAGTGAAAGCCGCCAAAAACTGGCGGAATGCTCTC48940.14830082826042887No Hit
GTCAAATAGGAAGAACTAGAAGATTCCACCTCTGGAAAGCGCCTACACCT48710.14760386891224947No Hit
GAACGGTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTG47440.1437554412070851No Hit
GCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGATGAGCCTGCGTAGT46950.1422706147696595No Hit
CGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGGCG45240.1370888735288476No Hit
GTGAAATCCGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCT44670.135361626448577No Hit
CGGAAAACCAGCTATATCCGATCTTGGTTGGCCTTTCACCCCTAGCCACA43060.13048291101132134No Hit
CCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTG42040.1273920478150476No Hit
CGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGT41360.1253314723508651No Hit
CCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCGGAGGCGC40720.1233921072081051No Hit
GCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAA40690.12330119946703821No Hit
GGCGGCCAAAGCCCGGGCTCTCGATACGCCCGTGGAACGCCGTCTCCCCG40500.12272545044028135No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAGATATCTCGTAT40410.12245272721708071TruSeq Adapter, Index 7 (97% over 36bp)
GCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGA40330.1222103065742357No Hit
GTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGATTG39880.1208466904582326No Hit
CGGTGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGAT39870.12081638787787698No Hit
GGAACAGCGGCTTAAGCCATTAGGTGTAGGCGCTTTCCAGAGGTGGAATC39670.12021033627076447No Hit
CGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCA39050.11833157628871573No Hit
CCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGGC37730.11433163568177322No Hit
GGGGAATCTTGGACAATGGGCGAAAGCCCGATCCAGCAATATCGCGTGAG37340.11314983504790382No Hit
GCCAGCGCTACTAATTGAGTGCCAGCACCTAGCTGTGCTGTCAGTAAGAA37190.11269529634256946No Hit
GCTGACATGAGTAACGAAAAATCCTGTGAAAAACACGATCGCCTGCCAGT36450.1104529053962532No Hit
GGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCG35720.10824081703029258No Hit
GTTTTTGGTGACAAGACCTGGAGATATCAGAAGTGAGAATGCTGACATGA35430.10736204219997947No Hit
GTTCATATTAGGGAAAGGAGAGTGAGGGGAAGAGGGGGCAGCCCTCGGCC35310.10699841123571194No Hit
GGCCTTTCACCCCTAGCCACAAGTCATCCCCGTATTTTGCCACATACGTG34650.1049984409322407No Hit
CGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCTGGGTCCCT34490.10451359964655071No Hit
GCCGAGTCAGAAAGGCTTTGATGACTCAAGGTTCATATTAGGGAAAGGAG33730.10221060353952319No Hit
CCCAGATAAACTACCCACCTCGCAGTGTCCCGCCTCCCCCCGAATGATCG33550.10166515709312196No Hit
GTCATGATCGCGCACTCGACGAAAGAGCTTTACAAGCGGCATTGCCCTTC33430.10130152612885446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)