FastQCFastQC Report
Fri 14 Jul 2023
SRR8763614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4877772
Total Bases243.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGATGATCTCGTATG1207122.4747364165442747TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGATGATCTCGTAT218660.44827843531842TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGGCGATGATCTCGTATGCCGT127090.26054928356634954TruSeq Adapter, Index 7 (96% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGATGATCTCGTATGCC120970.24800257166591633TruSeq Adapter, Index 7 (96% over 33bp)
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA100160.20533965097179613No Hit
GGAACGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGT99690.20437609629970405No Hit
GCCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGG96530.1978977287171274No Hit
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC88630.1817018097606858No Hit
GTTTGTGTTAACAGAGCAGCGCGCTTCCCCCCGCACGATCCGCGAACGGG75510.1548042835950512No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC71180.1459272799138623No Hit
GGTGAATCGGGTTGAAAGTGAAAGCCGCCAAAAACTGGCGGAATGCTCTC69340.14215506587843793No Hit
GTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGTG67730.1388543785974416No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGATGAACTCGTATG63320.12981336561036472TruSeq Adapter, Index 7 (97% over 35bp)
GTGAAATCCGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCT59050.12105936890859188No Hit
GGCGGCCAAAGCCCGGGCTCTCGATACGCCCGTGGAACGCCGTCTCCCCG58460.11984980027766777No Hit
GCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAA56350.11552405483487133No Hit
GGGGAATCTTGGACAATGGGCGAAAGCCCGATCCAGCAATATCGCGTGAG56250.11531904320251131No Hit
GCCAGTGGAAGGTTTTCTGCGTTCAGTCAATCTACGCAGAGTGAATCGGT54260.11123931171854691No Hit
CTGGAAGTTTCAACCTTGTTGACTATGACAACAGTCGCGACTATAAACAG51030.10461743599331827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)