FastQCFastQC Report
Fri 14 Jul 2023
SRR8763649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9068616
Total Bases453.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAGGAGATCTCGTATG634670.6998532080308616TruSeq Adapter, Index 19 (97% over 36bp)
GCCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGG306560.3380449673908345No Hit
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA294360.32459197743073476No Hit
GGAACGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGT290030.3198172686989944No Hit
GTCGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAG235490.259675787352778No Hit
GTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGTG211330.2330344564154001No Hit
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC173510.19133018753909087No Hit
GGCGGCCAAAGCCCGGGCTCTCGATACGCCCGTGGAACGCCGTCTCCCCG158440.1747124368260824No Hit
GTCAAATAGGAAGAACTAGAAGATTCCACCTCTGGAAAGCGCCTACACCT153470.16923199747348436No Hit
GCGGTTATGAGTACGACCGGGCGTGGACGGCATTCGGTCCTCCGGATTTT142540.1571794417141491No Hit
GCCGGCGATGCGCCCCGGTCGGATGTGGAACGGCGACGAGCCGGTCCGCC137210.15130202888731864No Hit
CCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGA134980.14884299875526763No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC122820.13543411695897148No Hit
GGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGAT120510.13288687049931325No Hit
CGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCA120370.13273249192600062No Hit
GGCGGTTGACGGCAACGTTAGGAAGTCCGGAGACGTCGGCGGGAGCCTCG109840.12112101780470141No Hit
GGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATACCGTCTGTGATCCAT109730.12099972035424149No Hit
GCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAA107030.1180224192974981No Hit
CGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGAT105330.11614782233584486No Hit
CCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAG104140.11483560446268759No Hit
GTCGGCGGGAGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCC101380.11179214116023878No Hit
CGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGT99120.10930002990533506No Hit
GGGGAATCTTGGACAATGGGCGAAAGCCCGATCCAGCAATATCGCGTGAG96830.10677483752757862No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAGGAGAACTCGTATG94640.10435991555933122TruSeq Adapter, Index 19 (97% over 36bp)
CCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTG93820.10345569820135729No Hit
GCCGCTACTAACGGGGTCTCGGTTGATTTCCCTTCCTTTAGCTACTAAGA93610.10322413034138836No Hit
GGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCCAA92780.10230888594246355No Hit
CCCGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGA91650.10106283031501169No Hit
GCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGATGAGCCTGCGTAGT90820.10014758591608687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)