FastQCFastQC Report
Fri 14 Jul 2023
SRR8763650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8763650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7063600
Total Bases353.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTACCATCTCGTATG524570.742638314740359TruSeq Adapter, Index 2 (97% over 36bp)
GCCGGAGGCGCGCGCTGCCTTCCACAATCGGGGAGACGGCGTTCCACGGG285570.40428393453762956No Hit
GGAACGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGT285550.404255620363554No Hit
GTGGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCC198520.28104649187383207No Hit
CGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAGA191790.27151877229741206No Hit
GTCGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAG178330.25246333314457214No Hit
GTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGTG166820.23616852596409763No Hit
CCCAGGCGGAGTGTTTCACGCGTTAGCTGGGCCCCTGATCCGCGTAGACC126510.1791013081148423No Hit
GGCGGCCAAAGCCCGGGCTCTCGATACGCCCGTGGAACGCCGTCTCCCCG121750.17236253468486323No Hit
GCCCGATCCAGCAATATCGCGTGAGTGAAGAAGGGCAATGCCGCTTGTAA121620.17217849255337223No Hit
GGGGAATCTTGGACAATGGGCGAAAGCCCGATCCAGCAATATCGCGTGAG120790.1710034543292372No Hit
GCCGGCGATGCGCCCCGGTCGGATGTGGAACGGCGACGAGCCGGTCCGCC117280.16603431677897956No Hit
GTCAAATAGGAAGAACTAGAAGATTCCACCTCTGGAAAGCGCCTACACCT108570.15370349396908092No Hit
CCCGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGA104640.14813975876323687No Hit
GCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGATGAGCCTGCGTAGT103440.14644090831870435No Hit
CCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGA99090.1402825754572739No Hit
CGTCAATTCCTTTGAGTTTCGGTCTTGCGACCGTACTCCCCAGGCGGAGT96070.13600713517186702No Hit
CGAATGGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCA90720.1284330936066595No Hit
GTGAAATCCGGAGATCTACGAAGGAACGCCAAAAGCGAAGGCAGCTCTCT89960.12735715499178887No Hit
CCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGTG89720.1270173849028824No Hit
CAGCTCTCTGGGTCCCTACCGACGCTGAGGTGCGAAAGCATGGGGAGCGA87910.12445495214904582No Hit
GGTGAATCGGGTTGAAAGTGAAAGCCGCCAAAAACTGGCGGAATGCTCTC87330.12363384110085508No Hit
CGGCGACGAGCCGGTCCGCCGATCGACTCGGGGCGTGGACCAGCGTGGAT86240.12209071861373803No Hit
GGGCAATGCCGCTTGTAAAGCTCTTTCGTCGAGTGCGCGATCATGACAGG84620.11979727051361913No Hit
GGAATTTCGTGTGTAGGGGTGAAATCCGGAGATCTACGAAGGAACGCCAA82010.11610227079676086No Hit
CCCCACTGCTGCCCCCCGTGGGAGTCCGGGCCGTGTCTCAGTCCCAGTGT77130.10919361232232855No Hit
GGGGAGCCCACAGGCCAGCGTCCGGAGCGCGCAGATGCCGAAGCACGCCG76290.10800441701115579No Hit
GTCATGATCGCGCACTCGACGAAAGAGCTTTACAAGCGGCATTGCCCTTC76210.10789116031485362No Hit
CTCTGTCTCACTCAAGTGAATTGGTTTCGAGAGCATTCCGCCAGTTTTTG75810.10732487683334277No Hit
GGACGCTTTCAGAGGCGAAAGGCCATGGGGAGATACCGTCTGTGATCCAT75630.1070700492666629No Hit
GCCGAACAGTTCGGGCCAAATCCTGAAGAAAGCTAAAAAGCGCTGTTTGA73190.10361572002944675No Hit
CCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCATGGGGAG73150.10355909168129565No Hit
GCTGACATGAGTAACGAAAAATCCTGTGAAAAACACGATCGCCTGCCAGT73080.10345999207203127No Hit
CTCCTCTGTCTCACTCAAGTGAATTGGTTTCGAGAGCATTCCGCCAGTTT71180.10077014553485475No Hit
GCGGTTATGAGTACGACCGGGCGTGGACGGCATTCGGTCCTCCGGATTTT71170.10075598844781698No Hit
CCACCCTCTAAGCCTAAGTATTCCTCAATGACCGATAGCGTACAAGTACC70950.10044453253298602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)